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Including some interview footage of Thomas Friedrich, me, and our UW–Madison Science colleague Ajay Sethi.

V3 ARTIC Network primers are working beautifully. Oxford Nanopore Thanks, Kainalu for helping us get RAMPART set up!

Building out a real time ARTIC Network analysis pipeline distributed across several networked nodes

We analyzed the 13 most recent WI #SARSCoV2 using a modified ARTIC Network script to generate consensus sequences in real time. Check out our report on LabKey: go.wisc.edu/8tf99z. With: Gage Moreno TCF lab Trent Prall oconnorlab

Our preprint describing #SARSCoV2 viruses isolated from a human ➡️ cell culture using Illumina and Oxford Nanopore seq technologies. We show virus consensus sequences can remain stable through at least two serial passages on Vero 76 cells. biorxiv.org/content/10.110…

Go Mitchell Ramuta !

I am so excited to share our work comparing the #SARSCoV2 outbreaks in Dane and Milwaukee Counties, WI! Main takeaway: despite being ~100km apart and subjected to the same physical distancing measures - these counties experienced very different outbreaks medrxiv.org/content/10.110…

Kainalu ’s preprint on automated slow pipetting is now out. A great, simple robot makes it easy to get ultra long Oxford Nanopore sequences without much training: biorxiv.org/content/10.110…

The preprint for our simple solution to automated slow pipetting is out now. Increase accessibility and throughput of your long read Oxford Nanopore sequencing efforts with SNAILS. Dave O'Connor oconnorlab biorxiv.org/content/10.110…

So this was kind of fun to write--we found a new virus in common marmosets! It's a pegivirus! Short tweetorial on our discovery of the Southwest bike trail virus, SOBV, a weird virus in a weird species that still pretty much no one really understands. pubmed.ncbi.nlm.nih.gov/33007921/ 1/

Congrats Anna Heffron, MD, PhD ! She's got a great string of tweets breaking down her findings if you're interested in novel virus discovery!

Mantis: flexible and consensus-driven genome annotation biorxiv.org/cgi/content/sh… #biorxiv_bioinfo

Pipetting reagents slowly helps maintain long DNA fragments. Trent Prall's team developed SNAILS, a low-cost ($165 USD) robotic pipette which improves read length & 'increases the consistency & throughput of nanopore sequencing' over manual methods bit.ly/3lhIkz8

It might be time for some controlled shearing Oxford Nanopore #nanopore #ultralong #genomics #dna

Consistent ultra-long DNA sequencing with automated slow pipetting; Oxford Nanopore #sequencing bmcgenomics.biomedcentral.com/articles/10.11…

Pipetting slowly, as quickly as possible with six SNAILS running simultaneously to create 12 libraries at once for Oxford Nanopore sequencing #nanopore #DNA #Sequencing #longreads oconnorlab

Hands-free preservation of a 2.1Mb read through SQK-RAD004 protocol by SNAILS. Sequenced on a Oxford Nanopore Gridion. Base-called length is displayed from Minknow. Still need to map/confirm independently but exciting nonetheless oconnorlab UW-Madison Graduate School

More sequencing with #SNAILS : generated a 173 kb N50 and retained a 2.7 Mb continuous, base called read through the Oxford Nanopore RAD004 protocol. oconnorlab #ONT #longreads