Just received Oxford Nanopore Ultra-Long DNA Sequencing Kit. Going to have some fun @GIH_UQ this year..

Despite of power outage during my ultra-long reads Oxford Nanopore sequencing, a decent data (>12Gb, N50 >100kb with few +1mb reads) was generated from my test run. Happy to replicate Circulomics's protocol for uHMW DNA extraction and Nanobind Library prep @GIH_UQ

⚠️🌊 MAJOR FLOODING occurring now in #Brisbane. Bris River at Bris City has just reached the major flood level (3.50m) on the high tide. A second major flood peak is expected on Monday morning's high tide around 8am. Details: bom.gov.au/qld/warnings/ Follow @QldFES advice.

🌧️Another day with widespread rainfall totals 300-400mm across #SEQ. Highest in the state at #UpperSpringbrook 530mm. Highest in Brisbane area at #BrackenRidgeRes 444mm. Rain easing today, however #QldFloods will continue for next few days. bom.gov.au/qld/warnings/

Lego sequel-II seems ready for some HiFi sequencing 😉😉 PacBio

My in-house HMW total DNA extraction protocol bit.ly/3N9cr8v works great with different plant species but recently I had to scratch my head a lot for one plant sample. Finally,tuned protocol for nutty 🌿sample and happy Oxford Nanopore MinION seq 14Gb N50 ~31kb in 24h @GIH_UQ

Venomics Reveals a Non-Compartmentalised Venom Gland in the Early Diverged Vermivorous Conus distans mdpi.com/1549924 #mdpitoxins via Toxins MDPI. Old work but appears as a good stuff.

Top HiFi, middle Duplex Kit14, bottom ILMN.

Finally managed time to share my in-house tweaked HMW total DNA extraction protocol from a problematic plant sample bit.ly/3ytxezt as a fork of my previous protocol bit.ly/3N9cr8v protocols.io

Innovative platform shortens elite beef cattle lines’ breeding time. #UQResearch from Prof Tim Mahony and Di Xia @GIH_UQ bit.ly/3R7ujUg

Checkout the first preprint from my lab. Replication stress signatures that stratify ATRi, Wee1i, PARPi,and HU forks using AI and Oxford Nanopore Exciting collaboration @MichaelABoemo @GIH_UQ Subash RAI Alexis Bonfim-Melo, PhD @McclellandLab @prclarke1 Pauline L. Pfuderer doi.org/10.1101/2022.0…

QubitFlex Thermo Fisher made my work so easy to quantify ~300 plasmid constructs @GIH_UQ. Now time for the RNA synthesis.. 🧬>>〽️〽️

My new set-up with RTX A6000 is now ready for rock and roll @GIH_UQ

P2solo- the youngest and the most powerful member of Oxford Nanopore family @GIH_UQ

Received replacement of our faulty #P2solo with fresh reagents Oxford Nanopore . Kicked-off control run @GIH_UQ and impressive starts 👇Many thanks to Mike Yarski and team for arranging a quick replacement.

AI based laser based pesticide and herbicide. No chemicals. Meet Ironman…

AI image recognition models are powering the world’s next agricultural workforce: Watch as these drones use multispectral color grading to determine the ripeness + sugar content of apples, then gently pick them:

🫁Flipping the script on profibrotic alveolar #macrophages! New research reveals remarkable plasticity and shows druggable re-programming, with potential to target profibrotic cells & develop novel therapies for #pulmonary #fibrosis!

Great work Penny, Simon, and team

Checkout our latest research Springer Nature in Nature Communications rdcu.be/eBqBI A high-resolution, nanopore-based artificial intelligence assay for DNA replication stress in human cancer cells. A collaboration with Mike Boemo’s team Subash RAI Alexis Bonfim-Melo, PhD Pauline L. Pfuderer