What you're missing matters. Come see for yourself!

At #nanoporeconf , Alexander Wittenberg will discuss constructing high-quality, chromosome scale, fully phased, reference pan-genomes, & breeding new varieties of fungal resistant plants.

Attend online for free: bit.ly/4a4RF5g

RNA sequencing is routinely used to analyse isoforms as part of biomarker discovery, yet most methods fail to represent isoform diversity.

Learn how nanopore sequencing overcomes this challenge by capturing entire isoforms, in single reads.

Learn more: bit.ly/4aZknpr

We have released some raw Oxford Nanopore data for benchmarking purposes, along with some basecalled outputs.

We use the subset of 20k or 500k for benchmarking tools and methods a LOT!

We thought they would be helpful to others too.

4/5kHz R10.4.1 & RNA004

gentechgp.github.io/gtgseq/

Viruses don’t sleep so why should we? Midnight sequencing protocols 🦠🧬 #H5 #HPAI #Viromics #Alaska #LondonCalling Oxford Nanopore UAA BioSciences ARTIC Network

Time to result matters in cancer.

This case study highlights the potential of Cecilia Yeung & team’s gene fusion reporting workflow that takes ~5 hours — ‘substantially faster than any current fusion detection assays used in clinical laboratories’.

bit.ly/44EE43D

DNAscent detects BrdU in Oxford Nanopore reads from cultured human cells allowing us to determine DNA replication dynamics on thousands of single sequenced molecules. Fantastic optimisation by Earlham Institute #TransformativeGenomics gave ultra-long reads with N50>120 kb 2/6

Delighted to share our pre-print identifying human DNA replication initiation sites on single molecules by Oxford Nanopore sequencing. Key findings 1/6
biorxiv.org/content/10.110…

Verma & team have developed a single-tube #nanopore sequencing-based panel to detect variants that influence TB drug metabolism. Read how this portable, low-cost method has the potential to personalise TB treatment.

Read more: bit.ly/3UffP7a

Join us at #nanoporeconf Clinical & biopharma day for the 'Rare diseases remagined' panel.
Prof. Joris Vermeesch, Nick Seddon, & Paul Arvidson will unveil next-gen approaches in genomics for early detection & precision therapies. Learn more: bit.ly/3UDPyRd #nanoporeconf

Alzheimer's and dementia impact millions worldwide, with 10 million new cases annually. Discover how long nanopore reads are shedding light on the underlying biology of these diseases, potentially paving the way for new therapies and prevention efforts. bit.ly/3Qsxxmq

Review paper out today Nature Methods on long-read #sequencing for #metagenomic : rdcu.be/dGeIj Great advancements on utility of Oxford Nanopore PacBio forming novel insights in diversity and resolution. Great work by Daniel Agustinho Yilei Fu Todd J Treangen
BCM HGSC Rice Computer Science

The Acinas Lab's (📷) goal is explore the #microbes that live within the Arctic #Qikiqtarjuaq . They've recovered 26 #metagenomes using the portable MinION device — providing a new insight into this unique microbiome.

Keep up to date here: bit.ly/4aSP672

Direct detection of ribonucleotides embedded in DNA molecules by Oxford Nanopore sequencing, based on ribonucleotides-induced alterations in basecalling features, currents, and dwell times: nature.com/articles/s4200… Genome-Instability&Human-Pathologies Lavinia Grasso

Don't miss this panel on 'Building a rapid, targeted, & responsive infectious disease system' at the #nanoporeconf Clinical & Biopharma Day! Join experts Dr. Inès Hassan, Dr. Chuck Cooper, Dr. Rahul Batra, & Heather Carleton for groundbreaking insights: bit.ly/4aWvUWi

Structural variants hold missing pieces of the genomic puzzle.
Only with nanopore sequencing can you study them accurately in large cohorts — providing new insight in the mechanisms of health and disease.

What you're missing matters.

Learn more: bit.ly/4ax21f0 #WYMM

Swapna Uplekar from FIND describing the improvements we’ve made to our Oxford Nanopore AmPORE-TB assay by rapidly implementing World Health Organization (WHO) catalogue version 2.

BDQ sensitivity increased to 69% (WGS 74%)

CLF sensitivity increased to 67% (WGS 78%)

Can’t wait to see the full results 💥

An update from our lab: The first Wales Gene Park double run of sequencing on the PromethION device has started this afternoon. Good to see all that green! Oxford Nanopore Cardiff University #DNASequencing #NGS #longread #10kbprotocol #Promethion

Our latest work on discovering the dark side of CRISPR-Cas9 using our IDMseq Oxford Nanopore sequencing method KAUST Research KAUST Smart-Health Initiative grateful for great collaborators at KAUST Peking University and Altos Labs
link.springer.com/10.1186/s12915…

Now that’s what I call a packed house! Thank you #ESCMID community and our fantastic speakers for an exciting symposium on Oxford Nanopore sequencing applied to long-read 16S, tuberculosis drug resistance, nanopore-only bacterial genome wgMLST, and the future of metagenomics

Think we might to need a bigger room at next year's ESCMID!

Great to see so many people interested in the transformative impact of #nanopore technology in clinical microbiology and infectious disease research.

What you're missing matters #WYMM