Hi Brian. 37C works fine as well. We decided to go with 30C to be able to compare curing efficiencies between pFREE and the temperature-sensitive version pFREE-RK2. And also to reduce activity of Cas9 a bit when it cures ON.

Congratulations to our very own Ida Lauritsen, Postdoc at DTU Biosustain, who last Friday was awarded with DTUs 'Young Researchers Award' along with 5 other Phd candidates 👏🏼🧬🥳 Read more: bit.ly/37sKyFr #PhD #Biology

Universal method to remove cloning plasmids in bacteria #CRISPR pFREE your cells rdcu.be/uF8V Andreas Porse

First day of the prokaryotic #SynBio hands-on EMBO Course embl.de/training/event. A super diverse and interesting group of people!

Moreover, the bug—at least the non-GM wt—likes salt & it is VERY sensitive to oxidative stress. Will it be suitable to host redox reactions?

Bacterial Genome Editing Strategy for Control of Transcription and Protein Stability. dlvr.it/Q2tQXb

Bacterial Genome Editing Strategy for Control of Transcription and Protein Stability. dlvr.it/Q2tl9X

[PROTOCOL]Bacterial Genome Editing Strategy for Control of Transcription and Protein Stability. #CRISPRi, N-end rule for protein degradation, CRMAGE, Synthetic Metabolic Pathways, Methods Mol Biol. 2018;1671:27-37. doi.org/10.1007/978-1-…

Early notice of the 3rd Edition of the very successful EMBO hands-on SynBio Course at the EMBL premises in Heidelberg in 2019. Stay tuned and sign up for updates in events.embo.org/coming-soon/in…