Leveraging engineered #Pseudomonas putida minicells for bioconversion of organic acids into short-chain methyl ketones by Ekaterina Kozaeva, Manuel Nieto-Domínguez, Max Stammnitz @maxstammnitz.bsky.social et al. ACS Synthetic Bio Nikel SEM Group DTU Biosustain #OpenAccess pubs.acs.org/doi/10.1021/ac…

CIFR (Clone–Integrate–Flip-out–Repeat): A toolset for iterative #genome and pathway #engineering of Gram-negative #bacteria—our latest contribution led by Filippo Federici & Francesco Luppino Nikel SEM Group DTU Biosustain #OpenAccess #SynBio sciencedirect.com/science/articl…

Amazing tool for multiple cycles of genetic engineering of Gram negative bacteria developed by Filippo Federici and Francesco, now fully available! Nikel SEM Group

Engineering a phi15-based #expression system for stringent #gene expression in #Pseudomonas putida—led by Eveline-Marie Lammens, Daniel Volke in a nice collab between Nikel SEM Group & Rob Lavigne's team nature.com/articles/s4200…

Congratulations Pablo Iván Nikel @pabnik.bsky.social on this great achievement! We are thrilled to congratulate our group leader, Pablo Iván Nikel @pabnik.bsky.social, for being elected Fellow of ASM! A well-earned recognition of your outstanding contributions to our field! 🥳

Check our newest preprint 👇!

Preprint alert🚨Systematic #engineering of synthetic serine cycles in #Pseudomonas putida uncovers emergent topologies for #methanol assimilation · bioRxiv led by Òscar Puiggené González #SynBio #Methylotrophy #CircularEconomy Nikel SEM Group biorxiv.org/content/10.110…

Super exciting work by Òscar Puiggené González from Nikel SEM Group on advancing #methanol use by Pseudomonas Putida by engineering #serinecycle variants! Many congratulations, excited to be a part of this !

Really happy to share that our work on constructing six glyoxylate / glycolate sensors covering a three order of magnitude concentration range is out now in Nature Communications ! 🔥 Read below to see how we developed the sensors and what they are good for 🥳. nature.com/articles/s4146…

New paper out from our group!

New paper out in collaboration w/ Helena Schulz-Mirbach from Max Planck Society and many more! Check Helena's thread for a detailed overview of the manuscript!👇

New paper out! Check our new CRISPR toolkit for Cupriavidus necator!

Seven Critical Challenges in Synthetic One-Carbon Assimilation and Their Potential Solutions—led by Giusi Favoino & Òscar Puiggené González Nikel SEM Group FEMS #Microbiology #Reviews #SynBio #Metabolic #Engineering academic.oup.com/femsre/advance…

Ins and outs for designing bacterial metabolisms for C1-assimilation, from a multilevel perspective, now pieced together in this review from our Nikel SEM Group! Check it out at academic.oup.com/femsre/advance…