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I agree. Solubilize lipids in C12E8. Add FC12 in amounts expected for protein. Test reconstitution using Biobeads and o/n dialysis.

Can someone tell me the contact name for person at GN Biosystems who was working with Martin on dialysis plate?

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Leginon modifications back to Anchi and Jim to incorporate into their distribution? Or do we have to remember this next upgrade?

Will be important to use TLC to characterize this MHJF prep. I'm curious to see if detergent exchange was complete.

maybe better to let Changki do this. He should have conditions to evaluate the sample. Important to run standards!

Most vesicles look ok. EYPC/EYPA seems best, maybe neg charge causes increased adsorption to grid. What happened with DOPG?

If the robot is not crashing the microscope, then why not let it go overnight. I don't think any harm comes of a vacuum crash anyway.

DMPC liposomes prepared by reverse phase look excellent

DMPC liposomes prepared by biobead removal of C12E8 look good. Egg yolk are ok. Others not so good. Try again.

p2a3 reconstitution looks like aggregated/precipitated protein. definitely no vesicles. but I think you got the recipe wrong as we discussed

sesame is great. image of sample matrix is very helpful.

Scientists accidentally create mutant enzyme that eats plastic bottles theguardian.com/environment/20…

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It was great to have the kids around, even attending some of the talks x.com/profLemieux/st…

Awesome display of cellular machines at work x.com/janetiwasa/sta…

After a year and a half of planning and a little help from my friends, the cryo-EM facility at NYU is finally collecting its first data set for me tonight. The first of many more to come.