Hat tip to Alex Reis Sean Halper Grace Vezeau Daniel Cetnar Ayaan Hossain Phillip Clauer for their great work!

What sequences & structures control mRNA stability in bacterial operons? Announcing a new bioRxiv with lead Daniel Cetnar that provides *clear* and *quantitative* sequence-structure-function relationships + biophysical models that explain *why*

ATTENTION #synbio: Design 1000s of non-repetitive genetic parts using the Non-Repetitive Parts Calculator. Ayaan Hossain Ayaan Hossain leads you through the step-by-step process for promoters, insulated RBSs, terminators, and toehold RNA switches: github.com/ayaanhossain/n…

This Week on Friday, September 4, 2020 at 2 PM PST We are Hosting Professor, Xun Tang. Talk Title "Modeling and control for molecular self-assembly and the construction of genetic circuits" Apply at BioCompute.Club

NEW PAPER: We uncover & quantify how the 5' UTR, intergenic, and 3' UTR regions control mRNA decay + develop predictive biophysical models. Big 👏🙇 to Daniel Cetnar who characterized 82+ operons using RT-qPCR to show clear cause-effect outcomes. pubs.acs.org/doi/10.1021/ac…

Congratulations to Penn State chemistry's newest graduate, Dr. Joshua Kauffman! Josh, who is a member of the Sen group (Catalyst), recently defended his thesis.

See our recent work at the Howard Salis #synbio lab. DNA sequence in --> transcription profile out. Validated on 17396 sig70 promoters. Use it to identify cryptic promoters in genetic systems or to forward engineering synthetic promoters. Work done by myself and Ayaan Hossain!

If you are tired of Wordle, my friend created a new word game called Neword! Try it here: purefit.life/neword/

Our manuscript on Promoter Calculator was published today in Nature Communications! Many thanks to Ayaan Hossain and Howard Salis for helping to complete the project. Debug genetic systems and forward engineer new promoter sequences. Read more here: nature.com/articles/s4146…

NEW PAPER led by Grace Vezeau! We rationally engineered synthetic riboswitches to carry out sense & respond for human biomarker proteins [mCRP and IL32-gamma] that indicate disease or infection. 16-fold activation without trial-and-error! nature.com/articles/s4146…

Designing synthetic mRNAs 🧬 for optimal stability and expression? Checkout our work led by Daniel Cetnar on biophysics infused machine learning approaches for delineation of mRNA degradation kinetics ⚡ out now in Nature Communications nature.com/articles/s4146…

This is a great paper from the Howard Salis lab. - Measure the decay rates of 50,000 mRNAs in bacteria. - Use biophysical models + ML to build models of mRNA stability. - Profit. And a good reminder of what's possible when one turns a biological problem into a sequencing problem!