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Biacore troubleshooting challenge. What do you advise here? We've made 3 changes & re-running now... For info - protein-protein interaction, SA chip in #Biacore 1K+, 2-fold dilution series in blue (darker = higher) but lowest and highest concs highlighted differently. #Cytiva

I'm really pleased to have this accepted in Cell Reports A great team effort. Another example of how to use Affimers to understand protein function in cancer. The Astbury Centre University of Leeds FBS Research Facilities Medical Research Council Wellcome cell.com/cell-reports/f…

My last practical session with our Biopharma students today. Including mAb purification & SDS-PAGE. Have you purified a good (enough) reagent? Is one buffer better than another? Enough capacity in 1 column? + & - DTT (2 images of each) Thank you students - it's been good.

Biomolecular interactions - our facility technologies. SPR, ITC and MST for affinity, kinetics, thermodynamics and stoichiometry info. Watch this space... #Dianthus NanoTemper

Solution measurements of size & shape. Refeyn mass photometry of M.Wt. - VLPs & proteins (left-hand pics). Refeyn Loving Fida also for smaller & unstructured molecules, aggregation assays, Ex488 & 280nm, automation... V. grateful to Fidabio for the loan. RadfordLab

Installation and training on our new Dianthus from NanoTemper Binding assays especially for tricky systems - membrane proteins, multi-molecular complexes and small molecule libraries. Many thanks Medical Research Council for funding. The Astbury Centre @ScienceLeeds #hugthebox

Dear users, it is time to sing up for Fidabio User Group meeting 2024! Few last speaking slots remain available, so if you want to make sure your research is seen, hurry up: eventbrite.dk/e/3rd-fidabio-…

You're gonna need a bigger rack. 122 SPR cycles for small-molecule titrations - 10+ hours run time. Successful week of binding assays using #Biacore

Loving the rapid size analysis from Fida 1, before an assay or troubleshooting issues after. A gallery of results. Good - large signal, low aggregation Not great - less signal than expected Oops - aggregation, low signal Thank you for the loan Fidabio RadfordLab

Lots of good ITC binding results in recent weeks. Disproportionately pleased with this though... #equilibrated #clean #ITC

Really pleased the facility could contribute lots of ITC binding results to this publication. Congratulations on your first group publication James F Ross.

Super sessions at the Fidabio user meeting in Copenhagen, seeing how Fida fits with the biophysics & structural biology tool-kit. Designing better assays workshops. Great talk from @aip_taylor, RadfordLab, using Fida & other tech to study protein aggregation pathways.

This is nice - hydrodynamic radius and molecular weight conversion. Especially useful when interpreting Fida measurements Fidabio Well, "apparent" M.Wt. - think about folding, shape and multimerisation too if the numbers don't match. fidabio.com/molecular-weig…

I'm proud of how the facility contributes to some UG and Masters practicals. Very pleased that someone nominated me for one of the Faculty partnership awards - surprised to actually get the award. Couldn't have done it without our facility and teaching lab technical staff.

Abstract submission for the British Biophysical Society Biennial Meeting 2024 is now open! britishbiophysicss.wixsite.com/swansea2024 Join us for three days of all things Biophysics - and for the first time in Wales! An exciting line-up of speakers, including this year's BBS awardees below:

Mixed week of binding results, so it's good to finish the week with a positive, to check the machine is working properly - ITC of Ca2+/EDTA binding.

Bob Schiffrin, Joel Crossley, RadfordLab and colleagues based The Astbury Centre and @ScienceLeeds identify dual binding sites in the outer membrane protein chaperone SurA. #chaperones, #NMR, #smFRET nature.com/articles/s4146…

Excited to have Neo detectors installed on our Fida this week. Extending the concentration range for size, shape, heterogeneity & aggregation measurements. Kinetics & binding assays also from Fidabio. Thanks Tom & Joanne Walter Many thanks Biotechnology and Biological Sciences Research for funding.

Are you interested in proteins as exciting, dynamic interacting species. Come and join us!   Places are limited so please register ASAP at sheena-radford-lab.uk/fida.php. (£25.00 registration only, including excellent catering).

Since 2009, we’ve used this account to share University updates, highlight research stories, and connect with the Leeds community far and wide. However, in recent years - like many others - we’ve experienced a huge drop in engagement due to developments on the platform.