I am very excited to share the latest technology from my lab. Proteome-wide identification of amino acid substitutions deleterious for protein function biorxiv.org/content/10.110…

Mistranslation + biochem assays + MS = variant interpretation across the proteome. Innovative idea by Ricard RodriguezMias and co-developed with @noncanonikyle, and many other contributors from Villen, Fields and Noble lab. MiRo: MistranslationRocks

Excited to share our work on high-dimensional #phosphoproteomics in yeast exposed to >100 environmental and chemical perturbations. This is one of the largest and most systematic perturbation-phosphorylation resources for any species. #TeamMassSpec (1/8)

Great study by Mario Leutert in Judit Villen 's lab with a high dimensional systematic phosphoproteome study across 100 different conditions. To my recollection, the best ever survey of early signalling responses of a cell, illustrating the diversity of possible signalling states

Are you interested in studying phosphotyrosine modifications on proteins and want to save time and money? Check out our (Judit Villen) automated approach to enrich phosphotyrosine peptides using a pY superbinder or antibodies coupled to magnetic beads! biorxiv.org/content/10.110…

Excited to share our newest #preprint: Multidimensional proteomics identifies molecular trajectories of cellular aging and rejuvenation. A great project with the Judit Villen Maitreya Dunham Matt Kaeberlein teams! 📄Read: biorxiv.org/content/10.110… 💾Explore: rlsproteomics.gs.washington.edu 1/9

Creative study design and comprehensive phosphoproteomics analysis reveal paradigms of aging. The authors make important observations of asymmetric cell division, daughter cell rejuvenation and caloric restriction for lifespan extension.

Here's what happens when you start working with a postdoc & scientist too cheap to buy reagents: Mario Leutert Ricard RodriguezMias Checkout our automated and cost effective phosphotyrosine enrichment protocol now in JPR! Judit Villen Villen Lab, UW pubs.acs.org/doi/10.1021/ac…

The method might be cheap, but the data is priceless.

Phosphotyosine modifications are done playing second fiddle to phosphoserine and phosphothreonine. Very excited about the cool biology this pY enrichment method will uncover! Congrats Judit Villen Alexis Chang Ricard RodriguezMias and Mario Leutert.

Really nice talk by Sophie Moggridge showing how to get better resolution in protein stability measurements than with circular dichroism. Not only that, she showed how to get confident measurements for >10 point mutants in one experiment.

Fantastic talk by Matthew Berg showing how he is uncovering fundamental tRNA biology while simultaneously building a great tool for probing biophysical properties of proteins en masse.

Well, one of my favorite papers (3rd fav!) of my career was just published at eLife - the journal. Here, we do what we do best: generate new understanding of the factors shaping multicellular evolution through a combo of synthetic biology, exptl evolution and theory. elifesciences.org/articles/84336

Are you happy with your current phosphotyrosine enrichment and measurement approach? If not, consider R2HaPpY! biorxiv.org/content/10.110… (1/2)

(2/2) We are excited to share our preprint for quick and cost effective phosphotyrosine peptide enrichment (R2HaPpY) that displays high sensitivity at low cost and effort. We include an SOP for easy setup, or inquire villenlab.gs.washington.edu/wordpress/inde… Villen Lab, University of Washington