Ultra‐high sensitivity mass spectrometry quantifies single‐cell proteome changes upon perturbation | buff.ly/3pqzimW #proteomics

Our scalable, quantitative and ultrasensitive LC-MS based workflow for single cell proteome analysis 'one cell at a time' is out! Single cells have a stable proteome but not transcriptome. Great collaboration with @Bruker,Evosep,Fabian Theis. Enjoy! bit.ly/3tgR2lB

Our review on "How ends signal the end" is out in Molecular Cell. Here we discuss various aspects of numerous terminal degron pathways and their regulation by E3 ubiquitin ligases. Review can be accessed for next 50 days via authors.elsevier.com/c/1ehF~3vVUPK7… Max Planck Institute of Biochemistry (MPIB) Jakub Chrustowicz

If you are a Ukrainian citizen and would like to look for a position in the field of proteomics, mass spectrometry or (bio)informatics in Vienna funded by us, you can contact me at any time. I would be happy if this content can be shared.

Our paper is out today in Science Magazine, I’m stoked OpenCellCZB ! Check out opencell.czbiohub.org: CRISPR tagging + 3D live imaging + IP mass spec to map the human proteome. science.org/doi/10.1126/sc… 👇 tweetorial from our 2021 pre-print.

A tribute to team science! Matthias Mann Lab Chan Zuckerberg Biohub Network Keith Andreas-David Brunner Preethi Raghavan Loïc A. Royer 💻🔬⚗️ Marco Hein @marcoyannic.bsky.social Shalin Mehta Jonathan Weissman's Lab Bo Huang Lab Hirofumi Kobayashi @heracanaj Dr. Rachel B + many other authors. So much fun to work with you all.

03/2022 Issue --> bit.ly/3iOvBn7 #SynBio, single-cell RNAseq of fly motoneurons, human kinase yeast array, #SynBio, p53 dynamics, plant #circadianclock... Cover (Uta Mackensen EMBO Press): #SingleCell #proteomics of the #cellcycle Matthias Mann Lab Andreas-David Brunner

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As single cell proteomics person, we always try to compare protein measurements to transcript, what if we can do both on the same cells? Great job by @cajun_science and collaboration between Environmental Molecular Sciences Laboratory (EMSL) and Cellenion biorxiv.org/content/10.110…

Together with Molecular Cell, we are celebrating their 25th anniversary by contributing a technology review highlighting cutting edge ´unbiased #spatial #proteomics with #single-cell resolution in tissues´ technologies. bit.ly/3xYtllu CPRatUCPH UCPH Healthscience Max Planck Institute of Biochemistry (MPIB)

Spatial transcriptomics is becoming increasingly popular, with differential studies coming in a lot. How should we compare multiple spatial images?

Feeling nostalgic! tomorrow is our last day The Francis Crick Institute- Was privileged to supervise a brilliant team since 2013, 7 postdocs are now PIs, together we published just above 100 papers :-) Maybe the Crick accepts this tweet as a final report in exchange for having no tenure programme?

Excited that our work on learning cell-cell communication in spatial transcriptomics is finally out Nature Biotechnology! Led by David Fischer & @AnnaCSchaar, we setup a graph neural network to estimate niche composition on expression in unbiased fashion. nature.com/articles/s4158…

1/n Cells in tissues are not independent. Yet, a lot of mathematical models deployed to explain gene expression of single cells assume independence - the i.i.d. assumption on observations. Can we use dependencies to understand gene expression variation better?

1/n Single-cell data analysis is complex with many tools to choose from - but which ones work best and should be used when? Led by @lukasheumos and @AnnaCSchaar, we wrote a free online book that aims to guide single-cell data analysts: sc-best-practices.org

Spatial-omics goes 3D🤗! Out at Cell Cell 👉🏼 We developed DISCO-MS with Matthias Mann Lab, a spatial proteomics technology for specimens fully imaged in 3D. DISCO-MS is aided by robotics and enables the study of diseases at their early stages. cell.com/cell/fulltext/…🧵👇

📢Ali Max Erturk Spatial proteomics goes 3D🤯.  Merging DISCO-clearing and proteomics to sample diseased tissues in their milieu during imaging promises to decode multifocal and/or multi-organ diseases. A must-read article from Harsharan Bhatia Andreas-David Brunner Furkan Öztürk Saketh Kapoor, PhD 🤩.

Congratulations Ali Ali Max Erturk, Matthias Matthias Mann Lab, Fabian Fabian Theis and teams for this tour de force study, brilliant! cell.com/cell/fulltext/…