Here, the highly talented team at SEMC present a step-by-step protocol for Smart Leginon Autoscreen that significantly reduces the cryoEM screening bottleneck, allowing for more rapid grid & sample optimization. Paper: biorxiv.org/content/10.110… Video: youtu.be/3WcI6WdzU8w

It is hip to be square! Check out our latest preprint. Amazing job from Eugene, Michael and Misha. Near perfect tiling in TEM avoiding double exposures is now possible biorxiv.org/cgi/content/sh…

1/ Today we are releasing OpenFIBSEM Version 0.2.2. This is a large release with new features, and fixes. Thanks to Lucile, Rohit and Hannah for contributing and testing this release. deMarcoLab github.com/DeMarcoLab/fib… See the thread below for the highlights:

OpenFIBSEM is growing. Thanks to a very dedicated team! More features to come and more support.

Another day a new instrument arrival. This time it’s not a EM but a Thermo Fisher MS. Looking forward to bringing #MassSpec & #cryoEM technological solutions to the community. SEMC - Providing #CryoEM expertise & resources for understanding molecular & cellular structure

Want to know a bit more about the New York Structural Biology Center, which I now direct? Take a look at this nice write-up from the Simons Foundation (Simons Foundation), which generously helps support the Center! annualreports.simonsfoundation.org/2022/new-york-… SEMC NCCAT Info

SEMC retreat at Tarrytown House Estate.

for those of you in the #volumeEM community and others spending time realigning your blocks on the ultramicrotome. With the help of our own Phil Francis Our first realease of Gizmo github.com/ehanssen/gizmo Bio21 Institute University of Melbourne #IanHolmesImagingCentre #IHIC

More automation and tools easier to use for FIBs. Working on TFS and Tescan. Go and try

Square beams square beams square beams. The article is finally out! doi.org/10.1038/s41592… Get yourself a square aperture and make your whole-cell tiled tomograms. Congratulation team! @eydchua Michael Alink, Misha Kopylov and Jake Johnston SEMC

CryoCycle your grids! Plunge vitrify & reuse clipped cryoEM grids. We show vitrified proteins & cells (thin areas), offering $10k+/yr in savings. Added benefits: simpler clipping, less grid damage, no frozen fingers, & no need for commercial plungers. biorxiv.org/content/10.110…

Hey cryo-addicted, a nice piece of work from the SEMC team at NYSBC. Bringing significant savings to our labs and making cryo-EM a little more environmentally friendly

If your time is shor to read the articles on square/rectangular beam here are the comments on @eydchua and Hamish Brown papers from Nature Methods news&Views nature.com/articles/s4159… deMarcoLab SEMC Bio21 Institute ARC CCeMMP University of Melbourne Science at Melbourne

SEMC Jing Wang @eydchua Aygul Ishemgulova Michael Alink Sharon Rozovsky Edward T Eng deMarcoLab Updated again: biorxiv.org/content/10.110…

Absolutely amazing collaboration with Tobias Mayer's group at Weill-Cornell. Kudos to Bo Gang and Jake Johnston for pulling this off. nature.com/articles/s4158…

This past weekend we received our Microscopy & Spectroscopy Arctis. This #cryoEM tool is part of our new tomography area to expand the biomedical research capabilities at the center. SEMC - Providing expertise and resources for understanding both molecular and cellular structures.

We used cryoET and biophysical approaches in vitro together with molecular biology in differentiated cells to show that chromatin compacted by PRC1-CBX8 is porous and accessible while PRC1 is dynamic in it. nature.com/articles/s4159…

2/ That was a great project led by Michael Uckelmann from the Chen Davidovich Monash Biomedicine Discovery Institute, together with Vita Levina, Cyntia Taveneau and others through a collaboration with the labs of deMarcoLab, Oliver Bell, Yann Gambin & Emma Sierecki EMBL Australia and Partha Das.

We are very happy to present our work on N-glycoproteomics!🍬 Our method enables the selective enrichment and precise quantification of intact N-glycopeptides to explore the dynamics of glycosylation microheterogeneity. nature.com/articles/s4159…

Interested in imaging protein suprastructures and membranes dynamics in situ? Are you "preoccupied" by the exocyst? Read our latest preprint where we time-resolved the choreography of a flexible exocyst higher-order structure and the vesicle in exocytosis biorxiv.org/content/10.110…