David Hoffman (@davephoffman) 's Twitter Profile
David Hoffman

@davephoffman

Sr. Staff Optical Engineer at @10xGenomics: 🔬+ 🧬. Views are my own (whose else’s would they be?)

ID: 400822398

linkhttps://david-hoffman.github.io/ calendar_today29-10-2011 16:40:37

439 Tweet

496 Followers

59 Following

10x Genomics (@10xgenomics) 's Twitter Profile Photo

If you currently own or have ordered a CosMx, we want to help keep your science moving forward. We are offering special promotions to help you transition to Xenium. Learn more: 10xgen.com/41OTXkG (5/5)

Talley Lambert (@talleyjlambert) 's Twitter Profile Photo

The nd2 package (Nikon NIS elements file reader for python) just got a major internal refactor. v0.6.0 reimplements the outputs of the official SDK in pure python. (No more wrapping of c code) Speed is improved, and it’s easier to install anywhere now. github.com/tlambert03/nd2

Alain Chédotal (@alainchedotal) 's Twitter Profile Photo

If you like building cool light-sheet microscopes, this is for you. The benchtop mesoSPIM @MesoSpim is faster, has better optics, larger field of view. Works for whole mouse, human cortex and more. Fabian F. Voigt @FritjofHelmchen @nvladimus biorxiv.org/content/10.110…

Adam Bowman (@adamb0wman) 's Twitter Profile Photo

Our paper on fluorescence lifetime voltage imaging of neurons is out! We combine kilohertz frame rate, micrometer spatial scale, and picosecond lifetime resolution in vivo. Check out the videos and original thread below. Cheng Huang science.org/doi/10.1126/sc…

10x Genomics (@10xgenomics) 's Twitter Profile Photo

What if you could explore 1,000s of your genes at subcellular resolution & get deeper cellular and spatial insights? What if you could do it with near-instant data after a run? Don’t wonder: find out how Xenium can take your ‘what ifs’ to ‘what next’. 10xgen.com/47blS2g

Jennifer Waters (@jencwaters) 's Twitter Profile Photo

If you are imaging more than one fluorophore in your specimen, be sure to perform the correct controls to ensure your results are not confounded by bleedthrough or autofluorescence! youtu.be/D_vxHMWC2mQ

Alex J Noble (@alexjamesnoble) 's Twitter Profile Photo

Muyuan has been working on this for some time, it's cool to see a manuscript: How to create full, atomic resolution scenes of biomolecules in Unreal Engine and fly around them in real-time. Really cool stuff with untapped potential. eman2.org/unreal_render biorxiv.org/content/10.110…

David Cook (@davidpcook) 's Twitter Profile Photo

Excited to share our comparison of Xenium and CosMx data! I feel like a lot of labs/core facilities have made similar observations internally, so we're really glad to put this out for the broader community. biorxiv.org/content/10.110… 1/n

Sadaf Shadan (@sshadan) 's Twitter Profile Photo

Cellular organelles exchange molecular information through transient contact sites, which are sensitive to experimental analysis. Reporting @nature, scientists use advanced microscopy to map and analyse dynamic subdomains at ER-mitochondria contact sites. shorturl.at/jAX39

nature (@nature) 's Twitter Profile Photo

Nature research paper: Motion of VAPB molecules reveals ER–mitochondria contact site subdomains go.nature.com/4bcisy8

Jonny Nixon-Abell (@abelljonny) 's Twitter Profile Photo

✅💡 very happy to have this paper finally out. Chris and I started working on this project in 2017…so great to get this across the line at last! nature.com/articles/s4158…

Nature Methods (@naturemethods) 's Twitter Profile Photo

We're very pleased to announce the February issue of Nature Methods, which includes a "mini-Focus" follow-up to our July 2023 Focus Issue on the future of bioimage analysis! (please see thread) nature.com/nmeth/volumes/…

We're very pleased to announce the February issue of Nature Methods, which includes a "mini-Focus" follow-up to our July 2023 Focus Issue on the future of bioimage analysis! (please see thread) nature.com/nmeth/volumes/…
Ruixuan Gao (@ruixuan3) 's Twitter Profile Photo

(1/x) We are excited to introduce VIPS (Volumetric Imaging of biological specimens via Photochemical Sectioning) in our preprint! VIPS uses a light-based process called “photochemical sectioning” to achieve petabyte-scale high/super-resolution imaging: biorxiv.org/content/10.110…

Eric Betzig (@eric_betzig) 's Twitter Profile Photo

Correlative two color 3D super-resolution single molecule localization microscopy and block face electron microscopy of mitochondria and the endoplasmic reticulum in vitreously frozen COS-7 cells doi.org/10.1126/scienc…

Michael Eisen (@mbeisen) 's Twitter Profile Photo

Here is the letter I submitted in response to the NIH's Request for Information on Maximizing Research Funds by Limiting Allowable Publishing Costs

Here is the letter I submitted in response to the NIH's Request for Information on Maximizing Research Funds by Limiting Allowable Publishing Costs