RNAseq is commonly performed. We compared N=30 wild-type Bl6 mice to an N=30 heterozygotic knockout with a phenotype, and then downsized. We found you need at least an N= 8-12 to avoid a >50% false positive rate. A 2nd line confirmed the finding. biorxiv.org/content/10.110…

Six years ago I wrote these cartoons to teach how to design experiments. The positive and negative control cartoons have >60,000 views. The Systems Validation cartoon only has 17,000 views - yet that one is necessary before you get to the controls. youtube.com/@experimentald…

Each year, the journal Skeletal Muscle's Impact Factor keeps going up. Now it's at 5.3 for 2-year impact, 5.0 for 5-year impact. It's the go-to journal for increases in understanding of molecular mechanisms underlying skeletal muscle biology. …eletalmusclejournal.biomedcentral.com

from Ramon y Cajal's "Advice to a Young Investigator":

A Critique of the Hypothesis, and a Defense of the Question, as a Framework for Experimentation academic.oup.com/clinchem/artic…

There are several papers noting GDF11 decreases cardiac hypertrophy. What those papers often don't mention is that it does so by inducing cachexia. ...so yes, the heart gets smaller, but so does the rest of the body, due to muscle wasting. cell.com/cell-reports/p…