We have a synthetic biology post-doctoral position available for capturing methane via an engineered archaeal construct (please see below).

Souvik Bhattacharyya PNASNews HarsheyLab Cool. We found motility was required for IS5 mutations that activate flhDC )(nature.com/articles/ismej…).

Congratulations, of course. But doesn't work with bacterial non-coding RNA regulators (e.g., anti-sense Sok RNA inhibiting Hok toxin mRNA of E. coli, doi.org/10.1002/j.1460…) predate this work by at least 7 years?

pnas.org/doi/10.1073/pn… Important question! Agreed: slow growth = spacer labeling from high copy number of pSCRATCH. Not clear persisters labeled/related recurring infections. Method relies on IPTG addition/active protein production (for Cas proteins) but persisters are dormant.

nature.com/articles/s4146… Amazing to see ribosomes protected/hibernating during stress in all kingdoms (E.coli to elephants...). Here, dimers, trimers, tetramers, and pentamers in yeast tethered to to mitochondria (!) after glucose starvation.

Vasili Hauryliuk I personally dislike the term abortive infection. Immune systems could be classified in 4 quadrants depending on whether they sense phage molecular patterns or a perturbation of host patterns, and whether they act on host molecules or act on phage molecules.

David Bikard I agree - 100-%. We do not see much death for things that one would call ”abortive”. It is a social construct ;)

Excellent agreement with our TAC story cell.com/cell-host-micr…

nature.com/articles/s4158… Never tire of the Laub group cementing the 1996 paradigm (doi: 10.1128/jb.178.7.2044-2050.1996) that toxin/antitoxin systems block phage.

Vasili Hauryliuk Hopefully your work will not have the word 'death' 22 times in it (without proof for prokaryotes)😉. Ah, will the 'Merchants of Death' ever stop making this unsubstantiated claim?

nature.com/articles/s4158… Even in single cells, persisters remain dormant with no translation. Great use of our tetracycline/rifampicin pretreat methods, which have been vetted 9 ways to ensure they create persisters. Would be nice if they mentioned the hibernized ribosome theory.

nature.com/articles/s4152… Beyond biofilms: Important discovery showing AI-2 regulates more than just biofilm formation. In Bacillus sp., synthesized AI-2 found to reduce sporulation by binding directly to sensor RapC, which then inhibits ComA activity to reduce sporulation.

pubs.acs.org/doi/full/10.10… 🙄Thru the looking glass: only in the persister field could authors claim “Eradication of P. a. persisters” but have 0.3% survival (Fig. 1be, the normal generation amount for persister studies), so they actually create persisters by stopping translation.

journals.asm.org/doi/10.1128/jb… Retro-research: Enjoyable read showing Las QS increases P. aeruginosa Psl transcription and Rhl QS increases Psl translation.

sciencedirect.com/science/articl… I'ing tripartite TAs IDed in Salmonella Gifsy-1 prophage (not called this but toxin is common HEPN protein & TAs are prevalent in prophages). No survival phenotype upon TA deletion (Fig. 1M), but less lysis from other prophages (due to slower growth?).

nature.com/articles/s4157… Just my opinion, based on what I consider sound research in a field with much noise due to studying dying cells, but please see the attached list of the usual errors from this lab in their Nature Rev Micro persister review.