
Pablo Carravilla
@carravilla_p
Scientist @ biofisika.org
Former @scilifelab @karolinskainst @MSCActions @Leibniz_IPHT @BiofisikaScienc
Membranes, biophysics, microscopy, viruses... and RNA!
ID: 1684083523
19-08-2013 19:53:45
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Continuing on the theme of dyes being tricksters! This paper is a must-read for those doing smFRET with cyanine dyes. Scott C Blanchard shows how dye photophysics, specifically excursions to the triplet state, can confound smFRET measurements. And how to fix it.





Long-term STED imaging of membrane packing and dynamics by exchangeable polarity-sensitive dyes hubs.li/Q02DRS-_0 Top-read paper from Biophysical Reports (Biophysical Society) - 6,323 downloads Leibniz-IPHT Pablo Carravilla Erdinc Sezgin


Special attention to membrane aficionados!! Thrilled to be involved in such a beautiful project!! Kudos Jennifer Kefauver Loewith lab, and the rest of the authors!!


This story by Kristin Böhlig is about making ether lipids observable. They differ in a tiny detail from regular glycerophospholipids: one side chain is ether- instead of ester linked. 20% of our lipids are ether lipids and we know very little about them. biorxiv.org/content/10.110…



Sharing thoughts on BCL2 proteins and how they dictate cell fate by tuning Mito-ER contacts. A new health meter? Extra puzzle pieces to understand AD, PD & cancer. Check out our latest perspective in Trends Cell Biology . 100% Ʌʗʜʋʗɑɼɼʘ . Congrats Alvaro! sciencedirect.com/science/articl…


VISION is now published in J Cell Science ! An easy-to-use software for biophysical image analysis designed by @luca_andronico & Florian Weber Ratiometric, multiparametric analysis+custom math operations on multi channels+cell segmentation+executable GUI! doi.org/10.1242/jcs.26…


🔥🔬"Measuring plasma membrane fluidity using confocal microscopy" in Nature Protocols🔬🔥 doi.org/10.1038/s41596… Thanks to the authors for the detailed protocol! Pablo Carravilla Jan Schlegel Ellen Sjule Franziska Ragaller Florian Weber Cenk Gurdap Taras Sych Erdinc Sezgin

Do you LOVE organelles?? Well I am THRILLED to announce that Nellie has been published in Nature Methods! Nellie a fully automated pipeline for organelle segmentation, tracking, and hierarchical feature extraction in 2D, 3D, timelapse, multichannel live-cell microscopy 🧵1/N