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Happy to share the cloning method that Michael Taschner developed over the past few years for heterologous expression of protein complexes. We've used it extensively. It may seem complicated at first, but it is actually easy, fast, & reliable. biorxiv.org/cgi/content/sh…

Make-or-break prime editing for bacterial genome engineering biorxiv.org/cgi/content/sh… #biorxiv_micrbio

Very happy to see this on BioRxiv! We developed make-or-break Prime Editing, through which we can make a wide variety of mutations. More in the thread below👇

Find out how we developed a Prime editing tool in which cells either make a precise genetic change or die, ensuring that only successfully edited cells survive. Given its high efficiency and precision, the applications it offers are endless! 👇🏼

Happy that the work has now been published mBio! Many thanks again to ᐯIᑕTOᖇ ᑎIᘔET for hosting me, Elisabet Bjanes Alex, Axel Janssen Paddy Gibson Afombravo Samira, Jonathon Baker, Ph.D. Andrew Varble & Swiss National Science Foundation scientific exchange grant for enabling! journals.asm.org/doi/10.1128/mb…

We're looking for an enthusiastic experimentalist PhD student to join our interdisciplinary group interested in the ecology & evolution of microbial communities unil.ch/mitrilab. Apply tinyurl.com/mu5swnk8 before Aug 31! Bonus: Lausanne is a beautiful place to live!

Logan is the largest dataset of assemblies. Rayan Chikhi et al. assembled over 27M SRA samples (>50 peta-byte of reads) into two peta-bytes of contigs. Amazing resource, fully open source! <3 📄biorxiv.org/content/10.110… Code: github.com/IndexThePlanet… 💾 registry.opendata.aws/pasteur-logan/

Introducing Bacttle! A board game targeting players in primary school that showcases the life of microbes. From basic concepts of environmental microbiology to motility & biofilm formation, the game aims to increase knowledge about bacteria. #JMBE: asm.social/20z

Happy to announce PneumoBrowse 2! Access it here: pneumobrowse2.veeninglab.com and read the preprint here: biorxiv.org/content/10.110…

PneumoBrowse 2 is freely available on pneumobrowse2.veeninglab.com. Many thanks to the entire lab for keeping track of updates and literature and specifically Axel Janssen Paddy Gibson Afombravo Vincent de Bakker Jelle Slager for putting it all together and Doran for IT support!

We are looking for a Network Manager for TARGet (Transdisciplinary Antimicrobial Resistance Genomics network). Apply by scanning the QR code

Dual CRISPRi-Seq for genome-wide genetic interaction studies identifies key genes involved in the pneumococcal cell cycle biorxiv.org/cgi/content/sh… #biorxiv_micrbio

Genome-wide dual CRISPRi-seq! Knocking down every gene with every other gene in the pneumococcus, identifying > 4000 genetic interactions (GIs), most of which have never been reported. biorxiv.org/content/10.110…

ICYMI: 3 new preprints from the lab: 1) Make-or-Break Prime editing: the most elegant bacterial genome editing tech to date 2) PneumoBrowse2.0: Revamped pneumococcal genome browser 3) Dual CRISPRi-seq: One of the most comprehensive genetic interaction study in bacteria ever

1) Make-or-Break Prime editing: the most elegant bacterial genome editing tech to date biorxiv.org/content/10.110…

2) PneumoBrowse2.0: Revamped pneumococcal genome browser biorxiv.org/content/10.110…

We are delighted to announce we NCCR AntiResist are funding a four-year PhD position with the Boeck lab in Infection Biology, focusing on pulmonary Pseudomonas aeruginosa infections using genomic and large-scale wet lab technologies. 👉Read more: nccr-antiresist.ch/join-us/

New job opportunity 📢 Associate professor, immunity/inflammation/infection (defined broadly) especially welcome Come and join a great community of supportive colleagues! Deadline 25 October, please spread the word path.ox.ac.uk/vacancy/associ…