Ten years ago, just after buying a house, I took a walk around my new neighborhood. Three young men were sitting on a loading dock on Park Street. One called out to me: "We're opening a brewery! We made beer! Do you like beer?" That was my introduction to Aeronaut Brewing Co. 1/?

"If there is no struggle, there is no progress. Those who profess to favor freedom, and deprecate agitation, are men who want crops without plowing up the ground, they want rain without thunder and lightning." – Frederick Douglass, 1857

Beshear '24

Over last decade or more, Dems were often told they had to sacrifice popular policy goals in order to preserve seats in swing districts, essential for Dem majority in house. Now Dems are willing to sacrifice swing seats in order to protect Joe Biden's unfounded belief that he

I know President Biden cares deeply about the future of our country. That's why I am asking that he declare that he won't run for reelection. My full statement:

“Donald Trump is on track, I think, to win this election. And maybe win it by a landslide and take with it the Senate and the House,” Democratic Sen. Michael Bennet tells me in a very newsy interview. “I think we could lose the whole thing and it’s staggering to me.”

Among many untenable things about this moment for Democrats, one tension is: 1. Claiming AMERICAN DEMOCRACY is on the line; and 2. Talking about Biden's decision like parents of an injured freshman debating whether he should try out for varsity tennis - "it's up to him,

Our inspiration was the famous ‘MEGA-plate experiment’ by Michael Baym, Tami Lieberman Roy Kishony et al, in which bacteria were grown on a set of antibiotic concentrations. Exposure to low concentrations facilitated resistance to higher concentrations. science.org/doi/10.1126/sc…

CXCL13 shows up prominently in T cells in autoimmune tissues and tumors. Human Tfh and Tph cells produce lots of CXCL13, yet its regulation is pretty unclear. With beautiful CRISPR screens by Jaehyuk Choi, Calvin Law, we found a few key factors... nature.com/articles/s4158…

Trump AG Bill Barr: “The Democrats have to stop their grossly irresponsible talk about Trump being an existential threat to democracy. He is not.” The truth: Trump is a threat to democracy. And Democrats should not be intimidated from saying so. thebulwark.com/p/truth-about-…

JD Vance has pledged he would do what Mike Pence wouldn’t - overturn an election and illegally seize power. He says the president can ignore the rulings of our courts. He would capitulate to Russia and sacrifice the freedom of our allies in Ukraine. The Trump GOP is no longer the

President Venn Diagram xkcd.com/2962

Epigenome editing is transforming biological sciences and medicine. We're grateful for the opportunity to publish this review of the field in Nature Biotechnology. Check it out here: rdcu.be/dPjsJ

Broad Clinical Labs is moving! Learn about our brand new building and our move schedule here. broadclinicallabs.org/broad-clinical…

Yay, thank you Addgene, our Fragmid resource is now available as a kit! addgene.org/kits/doench-fr… Paper here: pubmed.ncbi.nlm.nih.gov/38484704/ And prior explainer thread here: x.com/JohnDoench/sta…

Looks like my grant reviewers also write the instructions to assemble furniture

Let's talk a little more about paralog synthetic lethality. What's the best way to identify SL in a CRISPR double knockout screen? Rosalind Chou, grad student extraordinaire, looked through the different methods and saw pretty different results. So she compared them.

We’d like to announce that while we are not a hotel, we are a concept of a hotel.

Excited to see the revised version of our paper now published at Cell Genomics led by Yevgeniy Serebrenik cell.com/cell-genomics/…

Really excited to finally share this work from my internship at Microsoft Research in summer 2023! This project began when Judith and Kevin raised a simple question: can we develop better latent mappings of olfactory stimuli, such as molecules to odor perception? Summary (1/12):