
Darshini Poola
@darshini_poola
She/Her | Research Associate @GladstoneInst | BS-MS @IISERPune 2019-24 | Genomic Engineering | I write/sketch, sometimes | Bookworm @ tteok-book-i (link below)
ID: 1273318235690029057
http://tteok-book-i.medium.com 17-06-2020 18:17:16
49 Tweet
233 Takipçi
411 Takip Edilen

Rebecca Fang from Seth Shipman's lab presents her work on using PacBio technology to sequence the mitochondrial genome. Gladstone Institutes


New Preprint, New Retrons! More than a hundred of them. We synthesized a ton of bioinformatically predicted retrons and put them through a battery of tests, from RT-DNA production to editing in human cells, E. coli, and phage biorxiv.org/content/10.110… Gladstone Institutes UC San Francisco UCSF Bioengineering & Therapeutic Sciences

Multitrons: simultaneous multi-site editing of individual genomes using retron arrays, now published in Nature Chemical Biology Nature Chemical Biology: nature.com/articles/s4158…. Great job Alex González-Delgado and oso !

An explainer/behind-the-scenes: gladstone.org/news/new-one-s… via Gladstone Institutes.




Multiplexed editing of phage genomes using retrons, now published at Nature Biotechnology with lots of new bells and whistles. chloe kate crawford Santi Bhattarai-Kline Darshini Poola Karen Zhang Alex González-Delgado Matías Rojas Montero nature.com/articles/s4158…

New technology could lead to alternative treatments for antibiotic-resistant bacteria #NBTintheNews via Gladstone Institutes gladstone.org/news/new-techn…


🚨 NEW PUBLICATION 🚨 "Continuous multiplexed phage genome editing using recombitrons" Seth Shipman kate crawford chloe Nature Biotechnology UC San Francisco UCSF Bioengineering & Therapeutic Sciences Chan Zuckerberg Biohub Network go.nature.com/3yUNYTi


Gladstone Institutes Phage therapy could leverage retrons to overcome antibiotic resistance #NBTintheNews via Genetic Engineering & Biotechnology News genengnews.com/topics/infecti…

An experimental census of retrons, now published in Nature Biotechnology. We synthesized more than a hundred new retrons and put them through their paces, improved recombineering rates and precise editing in human cells up to 40%. nature.com/articles/s4158…

Again from Seth Shipman lab Gladstone Institutes, a compendium of retrons! nature.com/articles/s4158…


🚨 NEW PUBLICATION 🚨 "An experimental census of retrons for DNA production and genome editing" Seth Shipman Asim Khan Matías Rojas Montero Alex González-Delgado Nature Biotechnology UC San Francisco UCSF Bioengineering & Therapeutic Sciences Chan Zuckerberg Biohub Network nature.com/articles/s4158…

Have you seen this new publication from the Shipman Lab? Shoutout to the team and collaborators ⤵️ ⤵️ Seth Shipman Asim Khan Matías Rojas Montero Alex González-Delgado Nature Biotechnology UC San Francisco UCSF Bioengineering & Therapeutic Sciences Chan Zuckerberg Biohub Network nature.com/articles/s4158…

ICYMI: "An experimental census of retrons for DNA production and genome editing" in Nature Biotechnology nature.com/articles/s4158…

🚨New Pub: High throughput variant libraries and machine learning yield design rules for retron gene editors, now out in Nucleic Acids Res. This is a great one, and the last bit of kate crawford's PhD, who is off to David Bakar's Lab for a postdoc. academic.oup.com/nar/advance-ar…

Now published Nucleic Acids Res! SspA is a transcriptional regulator of CRISPR adaptation in E. coli. academic.oup.com/nar/advance-ar…