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Getting in early for #MicroscopyMonday with some #zebrafish tail fin neurons! From a (sort of) failed experiment, but hey at least it looks nice lol 🔴my weird Frankenstein-esque nes:hs-TdT knock-in; 🔵EGFP-rab11a knock-in from Bagnat Lab

#zebrafish peeps, has anyone ever injected capped mRNA (from IVT) into the larval yolk (~3 dpf) and observed translation of the transcript? If so was there any specificity/bias towards particular cell types/tissues? #AskZebrafish

So cool to see Sauron is shifting towards a career in science

Hey Christian Mosimann am I taking #AlwaysBeCloning a bit far? 😂

Turn your favourite beta-lactam antibiotic into a delicious meal in one simple step 🤌 carbenicillini 🤌

Prehatchling epaulette shark brain 🧠 Tricky dissection but super interesting anatomical organisation! Enormous telencephalon relative to the rest of the brain #devbio

What’s the best genus/species name, and why is it Boops boops?

Pretty impressive that they wrote this on an LB agar plate of all things

When you’re presenting at a conference and someone asks a difficult question

A tiny larval #zebrafish notochord dissected from the trunk 😮‍💨

Something nice arrived in the mail today ☺️ forever grateful to all my lab mates and the whole ARMI family for making my PhD journey so wonderful (Shout out to all the fish as well 🐟). I can’t think of many better places to grow as a scientist. Excited for what comes next!

Two asynchronously beating hearts in a very strange #zebrafish larva (larvae?) Thanks to Zebrafish Rock! for the technical assistance 😉

New #zebrafish transgenic line incoming ARMI 🤩 can anyone guess the dynamic structure?

It’s past midnight, everyone else has gone home and I FINALLY got the BAC recombineering I’ve been bashing my head against for a month to work 🥳 god I love #molbio (when it works)

Published today in nature, we describe an approach for single-molecule protein reading on Oxford Nanopore arrays. By utilizing ClpX unfoldase to ratchet proteins through a CsgG nanopore, we achieved single-amino-acid sensitivity. nature.com/articles/s4158…

#Zebrafish researchers, don't forget to fill out the ZFIN user survey before Oct 4th! It is such an incredible resource so please go help them out in retaining and attracting NIH funding. Link here: oregon.qualtrics.com/jfe/form/SV_9y… ZFIN Zebrafish Rock!

This year’s chemistry laureates Demis Hassabis and John Jumper have developed an AI model, AlphaFold2, to solve a 50-year-old problem: predicting proteins’ complex structures. Check out two examples of protein structures determined using AlphaFold2. First up, a bacterial enzyme

❤️ HMW ultrapure gDNA preps using a custom protocol, you could probably clone absolutely anything out of this stuff #molbio

Any #TEM experts able to weigh in on something? We are seeing these small electron-dense things in brain. Not sure if it's real (samples are from a storage disease) or artifactual, but it looks worryingly like lead precipitate... #SciTwitter #ElectronMicroscopy

Single-molecule live-cell RNA imaging with CRISPR–Csm go.nature.com/3EJPKsM