Inspired by previous work (e.g., by Prof Ana Pombo, Eric Betzig, HeilemannLab (👉🏻 heilemannlab.bsky.social)) we adopted Tokuyasu cryosectioning, widely known from Immunogold EM, to enable nuclear tokPAINT imaging of Pol II using TIRF. [3/11]

Achieving sub-3 nm localization precisions, tokPAINT enables detection of Pol II down to level of single antibodies. Bonus: cryosections are openly accessible to probe diffusion, enhancing imager binding kinetics, e.g. for molecular counting via qPAINT. [4/11]

Great news, this does not only work in fixed cells but also in ultrathin cryosections of tissues, as we show for tokPAINT imaging of Pol II within mouse tissues. [5/11]

Multiplexing with Exchange-PAINT can be also directly leveraged for tokPAINT as well as multi-omic cryosection protocols targeting proteins, DNA and RNA. [6/11]

Looking forward to my first time giving a talk in front of a biophysics audience Biophysical Society today. Come to ballroom B at 9:00 to see how I use chromosome walking in super-res to understand homologous chromosome communication. #BPS2024

Don't miss out on this important conversation! We hope you join us! Go to siesociety.org/events to register for free.

It was such as treat to have Wu Lab present at UNMC Dept of Genetics, Cell Biology & Anatomy, hear fascinating stories about the evolution of discoveries about homolog-pairing, and exchange deeply thought-provoking ideas. Thanks for your time. We had so much fun!!

Finally, we took a new lab picture with our newest members!

✨We're launching a Community Spotlights series to highlight some of the amazing people we've connected with through our work. We're thrilled to feature Dr. Shoumita Dasgupta for the first spotlight! Learn about Shoumita's work & her upcoming book: pged.org/community-spot….✨

🧬The Genome Organization & Nuclear Function Meeting (CSHL Meetings) kicks off today! 🧑‍🔬Our Structural Genomics Group Leader, Marc A. Marti-Renom, is 1⃣ of the discussion leaders, presenting: 🗣️"Sex-determining 3D regulatory hubs revealed by genome spatial auto-correlation analysis"

Join us to explore Genetics & Spirituality on May 22! Hosted by UNMC in Omaha + online. It’s been an honor working with Pastor Richard Randolph and co-organizers on this launch event for the Science and Spirituality Institute. Register to attend: scienceandspirituality.org/details-on-con….

Please join us in Kellogg 200 to hear the latest research from Dr. Ting Wu on Monday, May 20, 2024, at Noon. Geisel School of Medicine at Dartmouth Dartmouth 🌲 DartGRAD Dartmouth MCB Graduate Program Integrative Neuroscience at Dartmouth (IND) Dartmouth QBS BCBDartmouth Wu Lab

Fantastic work from mpsmela showing fast induction of #meiosis from human iPSCs! Happy I could contribute a bit to the work!

Our newest addition to lab (a fantastic technician) is super artsy, I love it! #FISH

WELCOME TO SMLMS2024🇵🇹 Happy to see so many shares and posts using the hashtag #SMLMS2024 Keep them coming!

our next talk features Jumana AlHaj Abed and her impressive work on chromosomal homolog interactions 🧬🔬 Jumana is also a new PI hiring, start applying now 😊 #SMLMS2024 Single Molecule Localization Microscopy Symposium

Our lab is on the lookout for curious and creative PhD candidates as part of the RNA Flagship at IIT. Don't miss out - there's still time to apply! SEMM IIT #compbio

Last week, #SMLMS2024 started! Considering we had an earthquake hitting Portugal just days before, we can say it was definitely groundbreaking. Hope you all had an amazing meeting! Looking forward to seeing you again at Bonn's #SMLMS2025 hosted by @UEndesfelder and HeilemannLab (👉🏻 heilemannlab.bsky.social)

The lab had fun at the HMS Genetics Department end of summer carnival! 🐰🐔🐤🦆🐑🐐

🚨New preprint from the lab🚨 🧬 What keeps certain chromatin domains anchored at the nuclear periphery? Our new genome-wide HiDRO screen uncovers a key role for RNA-binding protein hnRNPK. biorxiv.org/content/10.110…