"Protocol for assessment of CRISPR base editors and their components in Escherichia coli" Shelake RM, Kim J-Y. STAR Protoc. 2025-07-22/09-19. doi.org/10.1016/j.xpro…

"CRISPR-Dx and Metabarcoding Perform Similarly for Monitoring Mammals With eDNA on the Catchment Level in High-Alpine Ecosystems" Leugger F, Lüthi M, Schmidlin M et al,. Environ DNA. 2025-07-17. doi.org/10.1002/edn3.7…

これからのマラリア対策： ハマダラカのFREP1タンパク質のロイシン (L224) をグルタミン (Q224) に置換するだけで, マラリア原虫の感染を阻止できる。 この１アミノ酸置換を、CRISPRをベースにしたアレル・ドライブでハマダラカ集団に広げる crisp-bio.blog.jp/archives/39018…

新型コロナ後遺症の記事, 2025-06-26に更新しました crisp-bio.blog.jp/archives/26969… 新型コロナウイルスの厄介なところ： 感染しても無症状のまま (感染に気づかずに）済んでしまう場合がある。 無症状でも他人に感染させてしまう場合がある。 無症状でも後遺症に悩まされることになる場合がある。

FORUM "CRISPR/Cas-based personal glucose meters for nucleic acid detection" Li J, Lau CH, Zhu H. Trends Biotechnol. 2025-07-23. doi.org/10.1016/j.tibt…

"Controlling CRISPR-Cas9 genome editing in human cells using a molecular glue degrader" Khajanchi N [..] Saha K. Mol Ther Nucleic Acids. 2025-07-21. doi.org/10.1016/j.omtn… Cas9-degron (Cas9-d) adjusts Cas9 levels in hepatic cell lines, hiPSC & its GABAergic neurons.

Review "Multiplexed assays of variant effect for clinical variant interpretation" McEwen AE, Tejura M, Fayer S, Starita LM, Fowler DM. Nat Rev Genet. 2025-07-21. doi.org/10.1038/s41576… MAVE technologies and design (incl. CRISPR)

"RNA variant assessment using transactivation and transdifferentiation" Nicolas-Martinez EC [..] Gecz J, Jolly LA. PERSYST Investigator Team. Am J Hum Genet. 2024-07-30/08-08. doi.org/10.1016/j.ajhg… transactivation: #CRISPR-a

Review "CRISPR-Cas in actinomycetes: still a lot to be discovered” Mitousis L, Musiol-Kroll E, Wohlleben W. microLife. 2025-06-12. doi.org/10.1093/femsml…

A country has proposed reversing a 2009 scientific finding that man-made climate change puts human health and safety at risk. Any country seconds? cnn.com/2025/07/29/cli…

"Splice-site mutation enhances the efficiency of CRISPR-mediated gene knockout and new germplasm creation in fish" Xu Z [..] Tao W, Wang D. Aquac Rep 2025-07-24. doi.org/10.1016/j.aqre… Tilapia; Optimized single gRNA targeting SS resulted in exon skipping and intron retention.

"Phage-based delivery of CRISPR-associated transposases for targeted bacterial editing" Roberts A [..] Barrangou R. PNAS. 2025-07-25/29. doi.org/10.1073/pnas.2… Cas13a; CAST; DART (DNA-editing All-in-one RNA-guided CRISPR-Cas Transposase)

Review "CRISPR-Cas Based Platforms for RNA Detection: Fundamentals and Applications" Bagi M, Jamalzadegan S, Steksova A, Wei Q. ChemComm. 2025-07-21. doi.org/10.1039/D5CC03…

"Identification of regulatory sequences in Aca11 and Aca13 for detection of anti-CRISPR and protein–protein interaction" Song G [...] Tian Y. Nucleic Acids Res 2025-07-22. doi.org/10.1093/nar/gk… Aca (anti-CRISPR associated) Aca-driven protein–protein interaction detection (APID)