Excited to share a new preprint (biorxiv.org/content/10.110…) describing Precision Editing of Regulatory Sequences to Induce Stable Transcription-On (PERSIST-On), a hit-and-run strategy that enables robust and stable heritable gene activation in human cells. 1/15

Today we report in Nature Biomedical Engineering the eePASSIGE system, which uses evolved and engineered recombinases and prime editing to integrate large gene-sized DNA cargoes into the mammalian genome in an efficient, precise, and targeted manner. (1/13) drive.google.com/file/d/1WDMVqL…

Today we report in Nature Biomedical Engineering the systematic application of six recent prime editing developments to enable efficient and functional correction of CFTR F508del, the predominant mutation that causes cystic fibrosis (CF), in primary airway cells from CF patients.

We're excited to see our recent work on multi-functional transposons now in press. Nucleases and guide RNAs and catalytic introns, oh my! See the links and original thread below, for more... science.org/doi/10.1126/sc… drive.google.com/file/d/1fOVqT2…

Super excited to share that ENGRAM is out today! ENGRAM cells are programmed to write their histories into the genome, recording the intensity, duration, and order of biological events simultaneously. nature.com/articles/s4158…

Excited to share our study of T cell signaling, where we directed high-content, single-cell genetic screening to uncover how a single adapter protein orchestrates a complex cellular process essential for health. (1/14) biorxiv.org/content/10.110…

Our single-cell, pan-cancer analysis of open chromatin in human cancers, done in collaboration with TCGA and the Chang and Corces labs (among others including Illumina), is out in Science today. Congrats to Laksshman Sundaram and all authors! science.org/doi/10.1126/sc…

Honored to be included on the 2024 list of Innovators Under 35! Thank you Whitehead Institute David R. Liu Broad Institute for all of your support! Especially grateful for our fantastic team in the Raguram Lab (raguramlab.org)—stay tuned for more updates coming soon! (1/3)

Genomic tech dev is my favorite area of science, and today our paper describing our multiplex CRISPRa screening method to identify cell type specific regulatory elements is published! rdcu.be/dUnoq

Newest preprint from @EmilyAshkin in our lab! Critical roles of a STAG2 cohesin-PAXIP1/PAGR1 tumor-suppressive axis in lung cancer in vivo. Might transcend cancer type. Amazing collaboration with Zhichao Xu 徐志超 Jesse Dixon @HaiqingXu3@PetrovADmitri biorxiv.org/content/10.110…

Sammy Basso Sammy Basso, one of the most remarkable people to share our world, passed away last night from complications associated with progeria, the ultra-rare ‘rapid aging’ genetic disease Sammy both suffered from and transcended. Sammy realized that a base editing treatment

Today we report in Nature Biotechnology a system for the laboratory evolution of engineered virus-like particles (eVLPs) that enables the discovery of eVLP variants with desired properties, including improved production and delivery potency. 1/13 PDF: drive.google.com/file/d/1E6Nr86…

Excited to share the last piece of my PhD thesis published today in J. Am. Chem. Soc. where we engineer zwitterionic polymer modified LNPs to replace PEG and stabilize LNPs during nebulization, enabling efficient inhaled mRNA delivery: pubs.acs.org/doi/10.1021/ja…

Thrilled to see our work out in Nature Methods today! We applied prime editing to multiplexed dropout screens and benchmarked the tool’s ability to induce phenotypes when installing premature stop codons in essential genes.

Incredibly excited that our paper linking single molecule states of TF binding to gene expression using quantitative thermodynamic models is out in Nature today. An amazing collaboration with the Bintu Lab. Congrats to Ben, Michaela, and Julia! nature.com/articles/s4158…

WHO IS NAMING THESE THINGS

Interested in learning more about genome editing tool dev & their application to treat human diseases? 🧬 🏥 We're hiring graduating seniors who are seeking ~2 years of post-bac research experience. If you're interested in gaining experience in next gen medicines, please reach

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High-precision cytosine base editors by evolving nucleic-acid-recognition hotspots in deaminase bit.ly/46yTSaT

Today in Cell we report the use of prime editing to correct several mutations that cause alternating hemiplegia of childhood (AHC), a rare and devastating neurodevelopmental disorder, in patient-derived cells and in two mouse models. drive.google.com/file/d/1ibC50t… 1/10