SNPsaurus is now offering Illumina PE 150 sequencing of bacterial genomes: library prep, sequencing to 60X read depth, assembly, annotation, and alignment to a reference with variant calling--starting at $69/sample. See snpsaurus.com/illumina-bacte…

Good advice about types of artifacts from RAD methods. Defining loci "clusters" is ripe for artifacts...I think getting draft or better assemblies is a good way to avoid some artifacts that result from "flying blind" with a de novo reference. x.com/JonPuritz/stat…

The RAD-seq paper just passed 2000 citations. Great group of people involved--Nate Baird (now at Illumina) and Paul Etter (at SNPsaurus with me) worked through the details and Bill Cresko William Cresko gave insights and collegiality. Pleased PLOS One published it w/ wide readership.

With the new longer reads and higher output from PacBio we can pass the lower costs for de novo PacBio microbial sequencing along to you. Full service extraction, library prep and assembly/annotation informatics starting at $389/sample. snpsaurus.com/pacbio-small-g…

Olin Silander Bohdan Khomtchouk, Ph.D. PacBio Oxford Nanopore Indeed, it often makes sense to do PacBio microbial projects at service providers like SNPsaurus Institute for Genome Sciences University of Minnesota Genomics Center where the per microbe cost has come down significantly with multiplexing on the Sequel System

Very excited about upcoming access to PacBio Sequel II long reads! We have changed our pricing for genome sequencing projects (see picture for some example projects). Contact us at [email protected] to discuss your particular project. snpsaurus.com/pacbio-small-g…

Added HIFI accurate reads to our PacBio services. Bacteria starting at $300/sample; yeast $400, C. elegans $600. Rethink PacBio being for the final step of projects and consider it a single-platform solution for excellent genome information in single samples or populations.

New England Biolabs Q5 High-Fidelity DNA Polymerase is gaining ground on Phusion polymerase, according to citations in the literature. Visit labblab.com for insights like these and try our tools to identify which researchers are using different kits and products.

Interested in bacterial methylation with PacBio? Just add $25 per sample to get a boost in read depth to 100X and additional bioinformatics to find enriched motifs in the genome. Regular assembly/annotation starting at $300/sample and now methylation projects starting at $325.

We had several requests for extension - so now closing this Sunday (Jul 14 PDT)! calacademy.snaphire.com/jobdetails?aji…

We offer HiFi library prep and sequencing...ask us for pricing on your specific project snpsaurus.com/pacbio-small-g…

We were some of the original developers of RAD-Seq, but we think that for species with genome sizes under 600 Mb it now makes more sense to do whole genome genotyping (WGS at 10X read depth)--see some example project pricing below or at snpsaurus.com/whole-genome-g… #RADSeq

It is always fun to read the amazing research associated with samples sent to us for sequencing. Great paper!

The PacBio Sequel II just keeps getting better and our pricing just keeps getting better. PacBio bacterial genomes starting at $200/sample. Yeast $300/sample. A 1 Gbp genome for $1800. Library prep, sequencing, assembly and annotation included. snpsaurus.com/pacbio-small-g…

SNPsaurus is excited to offer a new PacBio service: full-length sequencing of individual plasmids for as low as $15/plasmid. No need to order primers to walk into a long insert. No charge if the plasmid isn't returned as a single sequence! snpsaurus.com/plasmid-sequen…

Even little Billy of the Family Circus wishes he could be doing some long-read genome sequencing with the PacBio Sequel, it appears!

This article hits a pet peeve of mine. It says "RADSeq" in the title but it uses the ddRAD method. This is important because the study is a diverse genus of moth. Not a diverse species, but 43 samples and 32 species. Why does that matter? 1/n

A surprising fraction of the plasmids we sequence have an unexpected assembly size. Here is a good example:

Today seems like a good day to announce some improvements we've had in the works: 1. We promised lower prices as our sequencing volume increased, and thanks to our wonderful customers, as of right now we are dropping our price from $25 to $15/sample! (1/6)

For bacterial genome sequencing, would you pick fast, good, or cheap? Now you can get ALL 3 with our new ONT sequencing service that almost always produces circular, full-length bacterial chromosome contigs with consensus accuracy >Q50 -- often with their full plasmids too! (1/3)