At the #MDCU Faculty of Medicine at Chulalongkorn University, Medical Biochemistry Conference on this week - great attendance by professors, researchers, Ph.D. and master's degree students. #Bioentist #Revolugen #bentolab #TANbead #Vazyme #Acrobiosystem #MAGTEC

FM magnetic Total Nucleic Acid (HMW-DNA + RNA) protocols in development Oxford Nanopore Illumina

Fire Monkey Spin columns & Magnetic Beads #HMWDNA #sequencing

#GenomicsThailand 2023 #bentolab #revolugen #Vazyme

Fire Monkey magnetic beads extract + Ligation Sequencing Kit V14/MinION Oxford Nanopore

100microLt whole blood, Fire Monkey magnetic, no size-selection post extraction, MinION LSKv14 Oxford Nanopore => ~8Gb and N50:40kb after 17hrs.

E coli Fire Monkey magnetic beads HMW-DNA extract captured on a precipitation star Oxford Nanopore

Miniaturized HMW-DNA extractions: 200microLt E coli culture + reduced Fire Monkey solution volumes on magnetic beads -> MinION/LSK114 Oxford Nanopore

Fire Monkey magnetic beads for mammalian samples. HMW-DNA or tuneable, miniaturized and automatable. No resting, fragmentation or size selection post-extraction. LSK114/MinION Oxford Nanopore performance.

In development. Miniaturized and automatable. Beads fragmentation of low and high molecular weight DNA input Oxford Nanopore PacBio Illumina

Breakthrough bead-based automated, #HMWDNA extractions for high throughput long reads. #oKtopure instrument using Fire Monkey chemistry & LGC magnetic beads generates 21Gb of raw data, N50 ~45kb on Oxford Nanopore LSK114/MinION without post-extraction size selection. #Sequencing

Results of collaboration with LGC demonstrating automated HMW-DNA extraction combining Fire Monkey chemistry + LGC magnetic beads + oKtopure liquid handling robot = superior HMW-DNA for short & long read #sequencing revolugen.co.uk/news/35/111/Re…

We've collaborated with RevoluGen to deliver a fully automated protocol that produces HMW DNA for Nanopore sequencing in under 2 hours, representing a meaningful advance in sample preparation efficiency for long-read #DNAsequencing: bit.ly/3IfbZVV #genomics

Fire Monkey chemistry + Dynabeads/Kingfisher Apex Thermo Fisher. 1ml E coli culture, on deck Proteinase K lysis to elution automated workflow. Raw data : 14Gb, read N50: 27kb on MinION/LSK114 Oxford Nanopore, single run.

In development: Low molecular weight DNA extract input fragmentation on Fire Monkey magnetic beads for long Oxford Nanopore reads

Fully automated Kingfisher Thermo Scientific Fire Monkey beads HMW-DNA extraction from white blood cells. No size selection post-extraction LSK114/MinION Oxford Nanopore

White blood cells extraction/fragmentation magnetic beads protocol. 460+ pores sequencing, N50:21kb Oxford Nanopore LSK114/MinION.

Single extraction/fragmentation protocol (magnetic beads) => single (no nuclease flushes) mammalian genome run. 21kb and 22Gb on a MinION flow cell, LSK114 Oxford Nanopore

Low Molecular Weight mammalian DNA input ➡️Fire Monkey beads size selection/fragmentation workflow ➡️appx. 30Gb on a single LSK114/MinION run Oxford Nanopore

NEWS We are absolutely delighted to announce our agreement with #ProteoQuest for the distribution of Fire Monkey HMW-DNA extraction kits across #China including Hong Kong and Taiwan. User-friendly, single extraction for both Illumina Oxford Nanopore reads. revolugen.co.uk/news/37/111/Re…