(15/n) Next time you think of Golgi remodelling, think both Mitosis and MIGAR!😊

(13/n) Finally, we asked why MIGAR is important during collective cell migration. We looked at cell migration behavior in MENA mutants. Loss of MIGAR in these cells leads to an incoherent intracellular reorientation and loss of directionality during migration.

5/n) After training our model, we were able to predict Golgi polarity in cells with ~75% accuracy with Chebyshev spectral convolution.

4/n) To this end, we developed a Graph Neural Network where each node represents a cell of the collective and the edges represent the interaction with its neighbors. The nodes are then classified into different class depending on the Golgi polarity in the cell.

2/n) In the collectively migrating epithelium, we found that the Golgi polarity showed three distinct orientations with respect to the direction of migration; Parallel, Perpendicular, and Antiparallel.

6/n) Our model also predicted a strong correlation between Golgi polarity and Orientation of Maximum Principal Stress. Further, we also found that the correlation operates locally and decreases progressively over multiple cell-lengths.

Hello Cell Migration Peeps, tune in on 15th Nov at 11 am ET to hear some wonderful science by Purnati Khuntia Purnati Khuntia (@tamal_das lab) and Prof. Nils Gauthier (@lab_gauthier) at our usual place 😉 If you are still not registered DM or sign up on cellmigrationseminars.com

In an exclusive #authorinterview with #biopatrika Purnati Khuntia from lab of Tamal Das at TIFR Hyderabad talks about her work on 'Golgi apparatus remodelling during wound healing' published in PNASNews. #wound #wound healing bit.ly/3vmTsB8

Heading into Collective cell migration..
Team work by Cell migration ladies, Rituraj Marwaha , Simran Rawal , and myself
Led by Tamal Das

pnas.org/doi/10.1073/pn… ... 2nd paper from the lab ... Here, we look at how Golgi moves from an apical to a basal position as a stationary epithelial cell starts moving during collective migration ... This amazing discovery was possible due to stupendous efforts by Purnati Khuntia

One of the most effective and sharp scientists I know!
Congratulations Purnati Khuntia ! Well deserved. Pretty sure you'll kick ass!

Fellow labmate Purnati Khuntia 's work gets published in PNAS! Collective Cellular Dynamics lab is on a roll! 👏 Tamal Das

Head over to the explainer below for a summary of the interesting new discovery, 'migration-induced golgi remodelling (MIGAR)'.

Purnati Khuntia Congratulations

We ventured a little outside our comfort zone and used Graph Neural Network (GNN) to understand spatial patterning of cell (Golgi) polarity during collective migration.

Purnati Khuntia Congratulations 🎉

Purnati Khuntia congratulations 🎊

In our group we are interested in understanding how organelles rearrange during collective cell migration as well as what roles they play ... watch out for this series ... wonderful stories by Rituraj Marwaha Purnati Khuntia Simran Rawal

Purnati Khuntia Congratulations, girl!!

Checkout this really cool paper from the lab 😎 .. Glad to be a part of this .. 😊

Purnati Khuntia Congratulations