Enhancing top-down proteomics of brain tissue with FAIMS | buff.ly/3p5ryED #proteomics

Alexey Nesvizhskii I think they tried (clumsily) to relate this instrument to a paper titled with the term "trueSCP". I felt that the use of this term in the paper was quite disdainful to other studies which paved the road to enable SCP.

There's (quite rightly) a lot of raving about the new AlphaFold DB resource today. Thought I'd weigh in with my own example. DNA-dependent protein kinase catalytic subunit (uniprot.org/uniprot/P78527) is an enormous beast - a single chain of 4,128 residues! (1/10)

Use of Linear Ion Traps in Data-Independent Acquisition Methods Benefits Low-Input Proteomics | buff.ly/3AYKdaL #proteomics

We are looking for another member to join our proteomics team! We are doing single cell and you can get your hands on all of the different techniques with all of the coolest toys ! #proteomics #postdocjobs #postdocs #academia #MassSpec bit.ly/30kxhP7

unsere ersten Daten zur Neutralisation von Omicron versus Delta sind fertig: 2x Biontech, 2x Moderna, 1xAZ/1x Biontech nach 6 Monaten 0% Neutralisation bei Omicron, auch 3x Biontech 3 Monate nach Booster nur 25% NT versus 95% bei Delta. Bis zu 37fache Reduktion Delta vs. Omicron

Researchers characterize the differences between single-cell spectra and bulk spectra by examining fundamental spectral features that are likely to affect peptide identification performance at low levels of input sample material. pubs.acs.org/doi/10.1021/ac…

Free, virtual top down proteomics meeting, with very interesting talks!

The @MacCossLab collaborated with MetaMorpheus @metamorpheus.bsky.social & others to reevaluate the current method of protein quantification in bottom-up proteomics. pubs.acs.org/doi/10.1021/ac…

Giving a lab meeting when you don't have any data x.com/KhoaVuUmn/stat…

What if the mass spec detector suddenly became 10x more sensitive? Proteomics of sub-microgram sample amounts now possible even on a high-flow LC @ 800 μl / min. Speed & sensitivity are now combined: Zeno SWATH SCIEX, DIA-NN. biorxiv.org/content/10.110….

Shotgun sequencing

Cold Denaturation of Proteins in the Absence of Solvent: Implications for Protein Storage (Barran) @PerditaB Emma Norgate #openaccess onlinelibrary.wiley.com/doi/10.1002/an…

As single cell proteomics person, we always try to compare protein measurements to transcript, what if we can do both on the same cells? Great job by @cajun_science and collaboration between Environmental Molecular Sciences Laboratory (EMSL) and Cellenion biorxiv.org/content/10.110…

Our FragPipe + DIA-NN 1.8.1 paper, making dia-PASEF truly sensitive, is finally out! 5.3k proteins @ 200 samples/day, 7.4k proteins lib-free from 10ng, 9k proteins - from 200ng. nature.com/articles/s4146…

We have an outstanding lineup of speakers for the JPrOS Webinar "Top-Down Proteomics", gathering online from 🇯🇵, 🇩🇪, 🇫🇷 @[email protected], and 🇺🇸 Mowei Zhou, Joe Loo! Starts at 14:30 in continental Europe and bright and early at 5:30 on the West Coast!　jproswebinar.com

Searching for a universal, bottom-up proteomics method? Max Perutz Labs Vienna performed a comparative survey of the 16 most common methods to provide a basis for selection preparation strategies in MS proteomics. pubs.acs.org/doi/10.1021/ac…

Very happy to share our new paper published in Angewandte Chemie, where we investigated single C. elegans nematodes by top-down proteomics! #nanoproteomics #microfluidics onlinelibrary.wiley.com/doi/10.1002/an…

Thanks to #projectDEAL, our new top-down nanoproteomics paper published in Angewandte Chemie will remain Open Access :-)

Acetic acid is a superior ion pairing modifier for sub-nanogram and single cell proteomics biorxiv.org/content/10.110… --- #proteomics #prot-preprint