Our latest article is out Nature Communications! We pulled apart single protein complexes from different directions and found a non-natural force-activated catch bond. Congrats Zhaowei Liu, Tinnefeld Lab and all co-authors! #biophysics #proteins #AFM #smFRET nature.com/articles/s4146…

1/4 Can you load lipid nanoparticles with a defined number of molecules? See our new approach for lipid vesicle sensing and cargo transfer on demand.  📢💡 onlinelibrary.wiley.com/doi/10.1002/an…

2/4 At the heart of the system is a single-molecule FRET-based sensor. It detects vesicle binding, making it possible to sense and engage with vesicles in real-time. This could have huge implications for understanding membrane interactions! 🧬

3/4 We pushed the boundaries by achieving targeted cargo transfer. A molecule first on the DNA origami sensor was 100% transferred to the vesicle. This could open new doors for targeted nanomedicine. 🚀

4/4 Congratulations to all the authors🥳 Ece Büber , Renukka Yaadav , Tim Schröder , Henri Franquelim and Philip Tinnefeld

Direct visualization of allostery in artificial DNA origami arrays or how we used a novel double FRET probe to study and engineer the coupling between antijunctions. Great cooperation with the labs of Yonggang Ke & Dongfang Wang now out in nature Comms nature.com/articles/s4146…

✍️📰Check out the press release from Universität München highlighting two of the latest articles from our lab, published in Angewandte Chemie and Nature Communications. lmu.de/de/newsroom/ne… onlinelibrary.wiley.com/doi/10.1002/an… nature.com/articles/s4146…

Advances in #nanotechnology: Teams led by #LMU chemist Philip Tinnefeld have published two studies showing how #DNA origami and fluorescent probes can precisely release molecular cargo. #chemistry #research lmu.de/en/newsroom/ne…

📝💭 Wiley Analytical Science Magazine has highlighted our recent papers. Read more here: Unlocking new frontiers in nanotechnology with DNA origami biosensors - 2024 - Wiley Analytical Science analyticalscience.wiley.com/content/news-d…💡

Join online Philip Tinnefeld at the BioPhotonics Conference, Oct 15-17 Photonics Media events.photonics.com/Presentation.a…

Check out our most recent work on monitoring the protective coating of single DNA origami nanostructures with a molecular fluorescence lifetime sensor: biorxiv.org/content/10.110…

New paper alert: This study presents a modular DNA origami single-molecule nanosensor that separates sensing from the signal output. A high FRET contrast enables tuning of the response window for improved sensor specificity, multiplexing, & logic sensing. nature.com/articles/s4156…

Researchers at #LMU have developed a strategy for precise, adaptable biomolecule detection using a #DNA origami scaffold. This flexible design could speed up diagnostic tool development. lmu.de/en/newsroom/ne…

Excited to share it now in its final form in Nature Nano: our approach to decouple sensing from signal output to build modular and tunable DNA origami sensors with high FRET contrast: rdcu.be/dZqUd 💡🧬🔬

Can we study DNA and DNA/protein conformations with Ångström/sub-second resolution in a fluorescence microscope? With GETvNA, we sure can! Check out our new paper in Nature Methods: nature.com/articles/s4159… Tinnefeld Lab Curious about how GETvNA works? 1/n🧵

1/5 DNA and DNA/protein conformations at Ångström/millisecond resolution—right in a fluorescence microscope! Check out our new GETvNA paper in Nature Methods : nature.com/articles/s4159… Want to learn about the science behind GETvNA?

2/5 ssDNA/dsDNA constructs are self-immobilized on graphene, with dsDNA standing perpendicular to the surface. By labeling specific bases, we can track their distance to graphene with a precision down to the Ångström scale, being able to detect subtle conformational changes.

3/5 We exploited this unique precision and well-defined DNA orientation to study bending due to A-tracts, bulges and the binding of the repair enzyme Endo IV, achieving 5° precision at 50 ms time resolution!

4/5 Huge congrats to the whole team! Big thanks to Alan Szalai, Giovanni Ferrari, Lars Richter for leading this work, and to everyone involved: Andrés M Vera, Izabela Kamińska, AksimentievLab , Kush Coshic, Ingrid Tessmer, Philip Tinnefeld . Exciting GETvNA applications on the way!

A team led by #LMU chemist Philip Tinnefeld has developed a new fluorescence microscopy method to visualize #DNA-protein interactions. This innovation could advance DNA #research, structural #biology, and biosensor systems. lmu.de/en/newsroom/ne…