Not so patiently awaiting this email 🙏🤞🙏🤞

Reviewer comments are positive 😃

The bacterial cell wall is important for their physiology but what are its impacts on their interaction with the host? How do we then take advantage of this to cripple pathogens? Check out our hot-off-the-press review article to find out more! Ivo Gomperts Boneca doi.org/10.1146/annure…

This single line on my Western has saved my project from sure doom

Not trying to start any enzyme wars here butttttt (template from Sketching Science)

Lipoprotein alert!!! Borrelia is wild

2x the gel means double the fun, right? 🤪

This week I was confused why my Western didn't work. I later realized it was because I completely skipped adding my secondary. That was a first for me, it's never too late to make new mistakes!

My birthday was Tuesday and someone already made me say my age out loud 🥴🫠

Terrabacteria: redefining bacterial envelope diversity, biogenesis and evolution Nature Reviews Microbiology from Simonetta Gribaldo nature.com/articles/s4157…

Does anyone have experience with myc-tag purification of proteins, particularly w/ detergent? My myc-tagged lipoprotein is almost completely in the flowthrough, very little binding. Sample is in 0.5% DDM for solubilization. Too much? Does myc purification tolerate detergent? 🆘🙏

📢📢📢 LIPOPROTEIN ALERT First description of LolAB in Bacteroidetes! Both not essential (‼️) but big effects on gliding, morphology, protein localization, & more! Also not interchangeable with E. coli LolAB despite structural similarities 🤯 Bacteroidetes physiology is WILD

Baby's first crosslinking experiment! 🔗 Left is untreated, right is formaldehyde-treated. How does it look?

Pride in Microbiology is excited to announce our first virtual LGBTQ+ STEM Day Symposium! Registration is open ~ abstracts for talks will be accepted through November 1. Learn about our the science in our community and socialize with our network!🔬 🏳️‍🌈 🔬forms.gle/8BYBrvEYQ1bAMz…

This year's #AgarArt Contest theme is "What Brings You Joy?" ASM invites you to express your happiest moments through the medium of agar—submit your masterpieces by 11:59 p.m. ET, Oct. 27, to participate! asm.social/292

Me in lab on a Friday when my multi-day protein purification fails

Harvesting big, gloopy cell pellets of stinky gut bacteria

ID & characterization of the lipoprotein N-acyltransferase in Bacteroides pnas.org/doi/10.1073/pn… Nicole M Koropatkin @[email protected] Shows deletion of Lnb negatively affects cellular growth & ability to degrade polysaccharides, deepening understanding of Bacteroidota & lipoproteins.

Making the jump to the new app 🦋 follow me there for more science and lipoprotein alerts!