Extra spooky edition of #FluorescenceFriday. Primary rat hippocampal neurons at DIV3 stained for F-actin 🟠 and phosphorylated myosin 2 🟣.

Wrapping up the week by visualizing the crazy axonal growth of dorsal root ganglia neurons in vitro using the SPY650-FastAct probe from Spirochrome! 🤩 Capturing one image every 10 minutes.

Since I missed #FluorescenceFriday this week I'll have to give it in person at #cellbio2023 ... Beach and Oakes Labs coming in hot.

Celebrating last night of ASCB with some BBQ🍻🍗 and great company 👩‍🔬! Carole Luthold Martial Millet Sasha Demeulenaere Joey Tidei Shreya Chandrasekar Maggie Utgaard Kotryna Vaidžiulytė Claire Leclech Romain Rollin @jfierro023

Massive 10+ year effort in lab spearheaded by Leonardo Parra-Rivas and Kayal (secret account 🤔). Unreal cover by Emma VIDAL that says it all.

Researchers grew rat neurons on a force sensor, which allowed them to discover that neurons develop tension after forming connections with other neurons, resulting in a contractile neural network. Without this contractility, neurons fail to fire. In PNAS: ow.ly/TQSj50QmtPO

It was really cool to learn some jython programming for this tool. It's great for exploring all the LUTs, and once you have a set of favorite LUTs I recommend to use @biovoxxel's plugin "LUT channels tool" from the biovoxxel update site! x.com/biovoxxel/stat…

The end of an era 😢 Melissa Quintanilla, the 1st PhD student to join the lab, has cleaned her desk and -20/-80 space, transferred her last data, and is ready to head to Paris to join the Piel Lab. What a pleasure it has been! Au revoir Dr. Quintanilla!!!

An interview with Patrick Oakes—Loyola University Chicago, IL, USA dlvr.it/T1zZxN

Quintanilla (Melissa Quintanilla), Oakes (Patrick Oakes), Beach (Jordan Beach) and colleagues Loyola University Chicago demonstrate subcellular biophysical mechanisms that enable myosin 2 filament assembly. hubs.ly/Q02l3klJ0 Matthew Akamatsu @Coronin @taraskalab #Biophysics #Cytoskeleton

Primary rat hippocampal neurons at DIV14 stained for Active-RhoA🔵and Phospho-Myosin Light Chain🟣. No AI required for this #FluorescenceFriday .

Actin retrograde flow in the lamellapodia of a stage one neuron. Happy #FluorescenceFriday !

Very happy to see the central Ph.D. topic (out of several) of Nikos Mougios in bioRxiv. He established a universal way to make all your antibody-based assays easily multiplexable by using erasable secondary #nanobodies. See it here: biorxiv.org/content/10.110…

Our April cover ➡️ hubs.la/Q02r6l130 shows overlayed temporal projection of actomyosin dynamics in the leading edge of migrating mouse embryonic fibroblast expressing EGFP-non-muscle myosin & mScarlet F-Tractin. From Melissa Quintanilla, Jordan Beach et al. hubs.la/Q02r6jGp0

Our actin in AIS plasticity story, led by PhD student David Micinski, is now online in Frontiers in Molecular Neuroscience. We like to thank our excellent reviewers whose comments and requests clearly improved the paper. frontiersin.org/articles/10.33… Thread below👇

Life has been pulling me away from the scope (I had a baby named Henry!), but #FlourescenceFriday never takes a break. CAD cell with a dual tag myosin 2 construct - green on the motor heads and magenta on the tails, giving a🟢🟣🟢triplet. Can even see a few processive runs🏃‍♂️

Really proud to see this paper finally out ! 🤩🎉🍾 Thank you to Journal of Cell Biology and the reviewers for their precious comments that definitely strengthened our work. See our previous thread for most of the details ! x.com/pwoakes/status…

Very fortunate to have access to an abundance of scopes to capture images like this. These are primary neurons treated with blebbistatin and stained for acetylated tubulin. Melissa Quintanilla iLUT again coming in HOT this #FluorescenceFriday

Our October issue is out! hubs.la/Q02R_lsP0 The cover shows a color-coded projection through time of confocal images of a mouse primary Th1 T cell expressing EGFP-Lifeact migrating in confinement. From Caillier Alexia, Patrick Oakes et al. (hubs.la/Q02R_xZC0)

CAD cells reign supreme! Congrats @chillinwithpfn1 and Bruno!!