Structural basis of Rho-dependent transcription termination (Preprint by our laboratory. Posted 08/28/22.) biorxiv.org/content/10.110…

After an all day imaging session on the confocal

"Here, we report the identification of Chlamydia trachomatis hypothetical protein CTL0286 as the chlamydial RNAP ω ortholog, based on sequence, synteny, and AlphaFold and AlphaFold-Multimer..predictions." (Collaboration with Fan and Wang labs.) biorxiv.org/content/10.110…

Water clock🤩

Uncovering structural ensembles from single-particle cryo-EM data using cryoDRGN nature.com/articles/s4159…

"Deaths caused by bacterial infections accounted for more than 1 in 8 global deaths in 2019, with five pathogens accounting for more than half of those deaths, an international team of researchers reported yesterday in The Lancet." cidrap.umn.edu/news-perspecti…

although I co-authored the recent review on EM in PDB link.springer.com/article/10.100… I want to disclaim that I did not write the part about ribosomes; the choice to spotlight the work by Richard H. Ebright Chengyuan Wang 王程远 that I co-authored is coincidental

Cardboard proteins #blender3d #geometrynodes #molecularnodes

Structural basis of Rho-dependent transcription termination (New paper by our laboratory.) nature.com/articles/s4158…

Ian M Mackay, PhD (he/him) My first published mention of Midichloria is in a paper released this week. My main reason for adding a Results section on "Identification of ω in other obligate intracellular bacteria" was to be able to model Midichlorian ω and mention "Midichloria." journals.asm.org/doi/full/10.11…

"The results reveal..fundamental mechanistic similarities in factor-dependent termination in the three domains of life: bacterial, archaeal, and eukaryotic."

Our new structure of archaeal FttA-dependent transcription termination complex

Here, we report the structure of Rof-Rho antitermination complex and discover that Rof is a novel regulator of Salmonella virulence and host cell invasion. A nice collaborative effort with Yanjie Chao biorxiv.org/content/10.110…

Nature research paper: Structural basis of Rho-dependent transcription termination

🚀 Exciting news! hLife's launching ceremony was held in Beijing on Nov 15th, 2023. This marks an encouraging step in bridging basic research with clinical applications. #research #health #happy #Harmony #medicine

"Here we report cryo-EM structures of transcription-translation complexes from E. coli at various stages of the translation elongation cycle.

Structures of transcription-translation coupling complexes at each stage of the translation cycle (Collaboration between our laboratory and the laboratories of Yue Gao, Jinzhong Lin, and Chengyaun Wang. Extensively expanded preprint. Posted 07/25/24.) biorxiv.org/content/10.110…

"Here, we reconstitute the complete active transcription-translation system,...trap intermediate coupling states, and determine...structures showing...snapshots for the dynamic reaction trajectory"

Structural basis of RfaH-dependent transcription-translation coupling termination (Collaboration between our laboratory and laboratories of Gregor Blaha and Chengyuan Wang.) nature.com/articles/s4159…

The translation-transcription coupling in Archaea