Links to the conference and the workshop websites... 7ictrdelhi.org/home 7ictrdelhi.org/workshop/crisp…

It was fun meeting and interacting with nearly 60 participants at the workshop on CRISPR technologies, offered yesterday at AIIMS, New Delhi. 7ictrdelhi.org

Three day hands on workshop on CRISPR mouse genome engineering technologies (GONAD and microinjection) began today at the Van Andel Institute, Grand Rapids, Michigan.

TWEETORIAL time! In new work, we identify required transcription factors for neural differentiation using a TFome-wide CRISPR knock-out library. Congrats to first authors @congyiliv and Chao Dai (from our lab) and Gorkem Garipler, PhD (from @estebanmazzoni lab). 👏👏👏

There is much more in the paper, which is available here (Open Access): nature.com/articles/s4146… This was one of the first ideas 💡that I had for a CRISPR screen (10+ years in the making) and so very happy to see this study published. Enjoy & happy holidays! 🎅🛷🛷🛷

This was one of the most successful workshops on CRISPR Genome Editing in Mice: GONAD and microinjection. More details in the link below. linkedin.com/feed/update/ur…

This is an 'exceptional technological advance' in turning on/off of gene expression at mRNA levels. Very impressive! nature.com/articles/s4158…

If scientists get ‘curious’ and follow-up why some of their experiments showed weird results—instead of thinking their experiments did not work—is when new unexpected (but potentially breakthrough) discoveries are made. You will see such an example in this tweetorial!

A new wave of CRISPR-delivery technologies? See link for info linkedin.com/feed/update/ur… tinyurl.com/mpu5nvsu tinyurl.com/kcx4r7um tinyurl.com/swznneew tinyurl.com/mt5r7w2v tinyurl.com/3b7hvnsh tinyurl.com/49dwcbzz tinyurl.com/46xaawk9 tinyurl.com/2s3vd2rd

CRISPR guide RNAs & DNA primers are widely used reagents in biomedical research. Are these reagents always pure, without any contaminants? Probably not! Here is our analyses of reagents procured from several major suppliers in Europe, Asia and USA. shorturl.at/ovUW1

A simplified method to find out if DNA inserted correctly in your CRISPR mouse 3 Steps: Isolate genomic DNA, enrich target region via Cas9 (cut & ligate adapters) & long range sequence. Shown here for AAV CRISPR KI mice: can be used for any CRISPRed mice tinyurl.com/4ct5y93v

This new (simplified) method is a new addition to other short- and long- range sequencing based approaches, like CRISPR-KRISPR and CRISPR-LRS ncbi.nlm.nih.gov/pmc/articles/P… genomebiology.biomedcentral.com/articles/10.11… x.com/CRISPRGuru/sta….

Sunday morning breakfast, ಮಸಾಲೆ ದೋಸೆ!

Sunday morning breakfast: Rava Idli (ರವೆ ಇಡ್ಲಿ) and Coconut-Peanut Chutney (ತೆಂಗಿನಕಾಯಿ-ಶೇಂಗಾ ಚಟ್ನಿ), all made from scratch.

Looking forward to speaking about "Emerging Technologies in Genome Editing" in two weeks at the ABRF 2024 meeting.

The Sky is blue and the Sun is bright now in Nebraska, before the eclipse. It will be partial here though!

Sunday Brunch: Jowar Roti (ಜೋಳದ ರೊಟ್ಟಿ) and Stuffed brinjal/Badanekayi Ennegayi (ಬದನೆಕಾಯಿ ಎಣ್ಣೆಗಾಯಿ)

I am excited to be speaking about novel CRISPR genome engineering methods at Discovery Europe 2024 conference, May 22-23, Basel, Switzerland. oxfordglobal.com/discovery/even…

Scientists working in core facilities make a huge impact to research output. National Cancer Institute's R50 funding opportunity supports core scientists. Do you want to learn more about R50? Join today's ABRF Town Hall zoom meeting at this link, 2pm EST. abrf.org/event/abrf-vir…