And we're finally on to sequencing- 50 multiplexed faecal samples being tested for presence of Sabin strain polio on one flow cell.

Hmm.. how to phrase this in the overwhelmingly positive Twitter manner.. "Delighted to be heading back to Vellore to give polio sequencing from poo another try thanks to the protocol going wrong last time 😁". Possibly should use more emojis...

We'll begin with the valiant effort to stuff everything into a suitcase again. Definitely going to work this time. I should probably add a hashtag too.. er.. #Suitcase (I think that's how this works- I'm sure @alis_njc will correct me otherwise)

Better do one of those selfies (as my work experience kids spent the entire week doing). Spent mins trying to get both the suitcases in and make my face less round. Achieved neither. Prob need an emoji: 🐠

One near heart attack later, after it looked like another failed protocol, we might actually have some polio genomes to sequence. Hopefully I won't succumb to library-prep related trauma first.

Anyone inferring more sequencing issues from my lack of tweets would be correct. Two weeks of troubleshooting, results due in the <distant> future. Real science is hard.. if this were a movie we would have obliterated several invading alien species with that amount of effort.

Polio sequencing trip episode 3: This time it'll work flawlessly. Off to Pakistan we go.

First trial of our remote training for direct detection of poliovirus from stool by nanopore sequencing today, with the Institut National de Recherche Biomédicale in DRC Oxford Nanopore Institut National de Recherche Biomédicale (INRB)

postdoc epidemiology / evolutionary biology role with our team working on #polio eradication. opportunity to be involved in the Endgame! Deadline 23 Feb. DM or email me if interested. Imperial School of Public Health MRC Centre for Global Infectious Disease Analysis Gates Foundation imperial.ac.uk/jobs/descripti…

Our group has an opening for a Research Associate focused on the evolution and epidemiology of poliovirus: imperial.ac.uk/jobs/descripti…

Filming now in session for our Direct Detection of poliovirus by Nanopore Sequencing (DDNS) protocol training videos Nick Grassly MRC Centre for Global Infectious Disease Analysis Gates Foundation with Alistair Moulton Black

Congratulations to #INRB Pathogen Genomic Lab - INRB team who stands as a key asset for Africa CDC Pathogen Genomics Initiative, expanding sequencing capacity to better monitor and control infectious disease #outbreaks in #DRC and beyond in the Central African region. @Central_RCC

Just arrived for #GrandChallenges 2022! Will be presenting poliovirus and NPEV collaborative surveillance work between Nick Grassly Pathogen Genomic Lab - INRB #INRB @NIBSC_MHRA Áine O'Toole and Andrew Rambaut. MRC Centre for Global Infectious Disease Analysis

It’s world #polio day and a wonderful opportunity to share our work on faster poliovirus detection with ⁦Bill Gates⁩ ⁦Trevor Mundel⁩ and Chris Karp Congrats ⁦Alex Shaw⁩ ⁦Placide Mbala⁩ ⁦Pathogen Genomic Lab - INRB⁩ and the polio sequencing consortium

Training in direct detection and nanopore sequencing (DDNS) for rapid #polio outbreak detection underway with the Central Public Health Laboratories in Oman. وزارة الصحة - سلطنة عُمان Nick Grassly @NIBSC_MHRA @MRC_Outreak Poliovirus Sequencing Consortium

Great progress on the first two DDNS sequencing runs with the Omani CHPL #polio team! وزارة الصحة - سلطنة عُمان Nick Grassly @NIBSC_MHRA MRC Centre for Global Infectious Disease Analysis Poliovirus Sequencing Consortium

Rapid analysis of DDNS poliovirus data using Áine O'Toole 's Poliovirus Investigation Resource Automating Nanopore Haplotype Analysis (PIRANHA) software running in ARTIFICE- from RNA to sequences in 2.5 days. وزارة الصحة - سلطنة عُمان Nick Grassly @NIBSC_MHRA MRC Centre for Global Infectious Disease Analysis Poliovirus Sequencing Consortium

And to finish off, the lab have performed near-whole genome sequencing of poliovirus in single reads using native barcoding. Great work! وزارة الصحة - سلطنة عُمان Nick Grassly @NIBSC_MHRA MRC Centre for Global Infectious Disease Analysis Poliovirus Sequencing Consortium

Dr Nuno Faria opening at CADDE Workshop on Portable Metagenomics for Pathogen Surveillance 2023 CADDE Project MRC Centre for Global Infectious Disease Analysis

Training for rapid DDNS sequencing of poliovirus at the Institut Pasteur de Madagascar with trainees from Côte d'Ivoire, CAR, Madagascar and Algeria Nick Grassly NIBSC_MHRA MRC Centre for Global Infectious Disease Analysis Poliovirus Sequencing Consortium