Fantastic preprint from Carl Wu, @successprocess, @rhodamine110 and company running the gamut of single particle tracking experimental design and analysis techniques to show formation of pre-initiation complex in cells: biorxiv.org/content/10.110…

phenomenal paper from Cai Lab. genome organization + nascent transcriptome + protein markers in single cells. I mean just wow. Also, as someone who reads a lot of these “kinds” of papers a lot, I appreciate the actual biological insights in this nature.com/articles/s4158…

This is such cool work dissecting the cell cycle using highly multiplexed imaging data. Not to mention a fantastic cast of scientists! @WStallaert Juanita Limas, PhD @dnatarheel Kate Kedziora

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Super impressed on first pass with DECODE for single particle localization. Colab notebooks made it super easy to get a couple of networks trained and predicting. Great work Artur Speiser Lucas Müller Jakob Macke Jonas Ries et al. biorxiv.org/content/10.110… github.com/TuragaLab/DECO…

Thanks for the shout out! This was a fun one to work and exciting story!

What an age we live in. Wanna see what alphafold2.0 does with your favorite protein? Copy paste run

DECODE uses deep learning for localizing single emitters in high-density 2D and 3D localization microscopy data. DECODE outperforms available methods and enables fast live-cell SMLM of dynamic processes. Srini Turaga ilastik Team and Anna Kreshuk's Lab Jonas Ries nature.com/articles/s4159…

The impact of the Janelia Fluor dyes by @rhodamine110 Jon Grimm @dyerfulchymist.bsky.social on the single-molecule fluorescence imaging field has truly been transformative, and the impact from their and HHMI | Janelia open sharing of these dyes have been even greater! elifesciences.org/articles/74981

Yoav Gilad It’s also a little funny because most of the money for Altos is coming from very strong VCs, who get much of their money from… university endowments.

The influx of single-cell data is staggering—not just in volume but in its multiple modalities. How can we optimally combine these modalities to make biologically meaningful predictions? (1/7) biorxiv.org/content/10.110…

This is just fabulous all around. Such valuable insight into how we view genome organization.

Unsurprisingly game changing work from Zhe J. Liu, Tjian + Darzacq Group and Bin Zhang unraveling the role of Brd proteins i genome organization: nature.com/articles/s4158…

I had the opportunity to see Christopher Obara from Lippincott-Schwartz Lab present this work lat week and it is absolutely bananas! I mean, I read a lot of papers from the single particle field and can’t think of many others that blown me away quite like this one. biorxiv.org/content/10.110…

A little late for Fluorescent Friday but here's some GIFs from our recent pre-print. Tracking single nucleosomes and putting them context of their local environment. We into 'Chromatin Density Classes' biorxiv.org/content/10.110…

I still remember when Wes first told me about funding coming through for this one, and being like “oh that’s gonna be rad” I’ve been fortunate to be a part of this all star team since then. I’m admittedly biased, but really think this one is hot shit!

Really elegant and frankly amazing work!

These dyes a in-freaking-credible! FR I've been fortunate to work with a bunch of cool imaging tools and reagents since getting into microscopy, these dyes take the cake. @rhodamine110 and co are magicians. biorxiv.org/content/10.110…

Absolutely amazing!

I trade you some habaneros for some figs.